The primary purpose of this document is to serve as a detailed description of the biological assessment programs for wadeable streams and rivers within U.S. Environmental Protection Agency (U.S. EPA) Region 1 states (i.e., Connecticut, Maine, Massachusetts, New Hampshire, Rhode Island, and Vermont). Specifically, this report concentrates on the target assemblages (e.g., benthic macroinvertebrates, periphyton, and/or fish) and the specific methods used by each state to determine whether biocriteria set for aquatic life use (ALU) are met in wadeable streams and rivers. The information contained in this report is critical to the eventual use of state data in assessing water resources on a national scale because it provides the necessary level of detail on New England state bioassessment methodologies in a single document. In addition, this report serves as a valuable resource for other states, tribes, and municipalities, both those developing bioassessment tools and those with existing programs.