Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Method for Concentrating Viruses Recovered from Sewage Sludges.
Author Berg, Gerald ; Berman, Donald ; Safferman, Robert S. ;
CORP Author Environmental Monitoring and Support Lab.-Cincinnati, OH.
Year Published 1982
Report Number EPA-600/J-82-228;
Stock Number PB83-117028
Additional Subjects Sludge ; Viruses ; Enteroviruses ; Methodology ; Reprints ; Sewage sludge ; Reconcentration procedure ; Katzenelson procedure ; Organic flocculation procedure
Library Call Number Additional Info Location Last
NTIS  PB83-117028 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 06/23/1988
Collation 6p
Buffered 10% beef extract eluates of primary, activated, and anaerobic mesophilically digested sludges were concentrated 20-fold by the Katzenelson organic flocculation procedure after diluting the beef extract in the eluates to a final concentration of 3%. The weighted mean recovery of virions from the concentrates was approximately 58% of the numbers present in the unconcentrated buffered 10% beef extract eluates. Flocculation of eluates that contained buffered 10% beef extract at times produced poor flocs. Application of the Katzenelson procedure to the diluted buffered 10% beef extract procedure for recovering virions from sludges permits an economy in the numbers of cell cultures required for assays of virions in beef extract eluates that is necessary in most laboratories.