The manual represents a procedural guide for conducting embryo-larval early life stage (ELS) toxicity tests with Japanese medaka (Oryzias latipes). These procedures are based upon evaluation of published papers and recent methods development work conducted at a laboratory in Duluth. The published papers are referenced in the appropriate places throughout the text of the report. If more detailed information on test apparatus or specific biological and chemical methods is desired, the reader is encouraged to study the reference material or contact one of the authors of the manual. All routine methods not covered in the procedure (physical and chemical determinations), should be followed as described in Standard Methods for the Examination of Water and Wastewater (3). It is suggested that those routines dealing with handling of fish not covered in the procedure should be followed as described by ASTM (4).

"EPA/600/3-91/063." "December 1991." "Environmental Research Laboratory-Duluth, Office of Research and Development." Includes bibliographical references.