Fractionation of particle emission extracts captured from complex combustion mixtures was performed upon environmental samples using an ion-exchange technique. Captured emissions from hazardous waste, municipal and medical/pathological incinerators along with urban air impacted by woodsmoke and mobile sources were utilized to evaluate the fractionation scheme. Separation of complex mixtures into class fractions is necessary to perform in-depth biological testing as well as to allow for efforts to identify biologically active components. A non-aqueous ion-exchange technique using the resin AG-MP1, allowed for fractionation of complex mixtures which potentially contained significant amounts of organic acids. Results indicated that when the resin technique was utilized in a solid phase extraction procedure, mass recovery found in the base/neutrals, polar/weak acid, weak and strong acid fractions of select marker standards was in the range of 60-118%. Mass distribution and recovery of eight combustion extracts indicated that 54-106% of loaded mass was recovered overall, with the base/neutral and strong acid fractions containing the majority of the mass. Mutagenic activity from the fractions was recovered in a range of 20-132% as compared to the whole extracts with the base/neutrals and strong acids responsible for a significant portion of the total mutagenicity.

"Presentation at 1991 EPA/A & WMA symposium "Measurement of Toxic and Related Air Pollutants", Durham, NC, May 1991." EPA/600/D-91/281. PB92-126879. Microfiche.

EPA/600/D-91/281.