| Main Title |
Direct viable counting method for meauring tolerance of aquatic microbial communities to Hg(2+1) / |
| Author |
Liebert, Cynthia. ;
Barkay, T.
|
| Other Authors |
|
| CORP Author |
Environmental Research Lab., Gulf Breeze, FL. ;Technology Applications, Inc., Gulf Breeze, FL. |
| Publisher |
U.S. Environmental Protection Agency, Office of Research and Development, Environmental Research Laboratory, |
| Year Published |
1988 |
| Report Number |
EPA/600/J-88/351; CONTRIB-628 |
| Stock Number |
PB89-237176 |
| Additional Subjects |
Aquatic microbiology ;
Mercury ;
Toxic tolerances ;
Cells(Biology) ;
Escherichia coli ;
Measurement ;
Cell division ;
Acid treatment ;
Metals ;
Thymidines ;
Fluorescence microscopy
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB89-237176 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
8 pages : illustrations ; 28 cm |
| Abstract |
Direct counts of Hg2+ resistant cells in natural waters were obtained by fluorescence microscopy after incubation for 20 hours in the presence of a growth substrate, nalidixic acid to halt cell division, and inhibiting concentrations of Hg2+. The method discriminated Hg2+ resistant from sensitive Escherichia coli strains. Estuarine samples were used to compare this procedure with two other toxicity measurements that determine the effect of Hg2+ on colony growth and on rates of radioactive thymidine incorporation into cellular material. Toxicity measurements based on direct viable counts and thymidine incorporation rates had comparable sensitivities, and both were 3-4 orders of magnitude more sensitive than the method that utilized colony counts. Thus, the direct enumeration of Hg2+ resistant cells is useful for predicting the potential of aquatic communities to sustain heterotrophic activity, an essential microbial process, in the presence of Hg2+. |
| Notes |
"Reprint article published in Canadian Journal of Microbiology, 34:1090-1095 1988." Caption title. "EPA/600/J-88/351." Microfiche. |
