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Main Title Application of a plant test system in the identification of potential genetic hazards at chemical waste sites /
Author Gill, B. S. ; Sandhu, S. S. ; Backer, L. C. ; Casto, B. C.
Other Authors
Author Title of a Work
Sandhu, S. S.
Backer, L. C.
Casto, B. C.
CORP Author Health Effects Research Lab., Research Triangle Park, NC. Genetic Toxicology Div. ;Environmental Health Research and Testing, Inc., Research Triangle Park, NC.
Publisher U.S. Environmental Protection Agency, Office of Research and Development, Health Effects Research Laboratory,
Year Published 1991
Report Number EPA/600/D-91/275
Stock Number PB92-124551
Additional Subjects Toxicity ; Mutagens ; Plants(Botany) ; Superfund ; Bioassay ; Water pollution ; Air pollution ; Pesticides ; Risk assessment ; Hazards ; Reprints ; Chemical waste sites ; Cleanup ; Micronucleus test ; Soil contamination ; Mutagenicity tests
Library Call Number Additional Info Location Last
NTIS  PB92-124551 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 11 pages ; 28 cm
The authors utilized the Tradescantia micronucleus (Trad-MCN) assay for evaluating genetic hazards at a chemical waste site contaminated with agricultural insecticides scheduled for clean-up under the Superfund program. The chemical analysis of soil samples from the site indicates presence of lindane (17 mg/kg), beta BHC (13 mg/kg), and heptachlor (0.4 mg/kg) in the subsurface sample. Tradescantia plants were planted at five locations to evaluate the mutagenic effects of the total environment, i.e., soil, water, and air. In addition, stem cuttings were also placed at these locations to sample the genetic impact of vapor phase organics in the atmosphere. The surface and subsurface samples were obtained from these locations for their chemical and biological analysis in the laboratory. The results of the Tradescantia planted on the site, as well as the stem cuttings exposed on the test site, showed significantly higher frequencies of micronuclei from contaminated plots before remediation; but no genetic activity was detected after the remedial action. The plants exposed to the soil samples in the laboratory yielded nonsignificant results except for one subsurface sample before remediation and two surface samples after remediation.
"Published in American Society for Testing and Materials, Plants for Toxicity Assessment: Second Volume, STP 1115, pp. 309-317, 11/91." EPA/600/D-91/275. PB92-124551. Microfiche.
Contents Notes