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Main Title Induction of Micronuclei by X-radiation in Human, Mouse and Rat Peripheral Blood Lymphocytes.
Author Erexson, G. L. ; Kligerman, A. D. ; Bryant, M. F. ; Sontag, M. R. ; Halperin, E. C. ;
CORP Author Environmental Health Research and Testing, Inc., Research Triangle Park, NC. ;Duke Univ. Medical Center, Durham, NC.;Environmental Protection Agency, Research Triangle Park, NC. Mutagenesis and Cellular Toxicology Branch.
Publisher c1991
Year Published 1991
Report Number EPA-68-02-4456; EPA/600/J-91/269;
Stock Number PB92-113315
Additional Subjects X rays ; Micronucleus tests ; Lymphocytes ; Humans ; Rats ; Mice ; Cytochalasin B ; Species specificity ; Chromosome aberrations ; In vitro analysis ; Dose-response relationships ; Phytohemagglutins ; Reprints ;
Library Call Number Additional Info Location Last
NTIS  PB92-113315 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 8p
The study compared the radiosensitivity of human, rat and mouse peripheral blood lymphocytes (PBLs) by analyzing micronuclei (MN) in cytochalasin B-induced binucleated (BN) cells. For each species and dose 4-ml aliquots of whole blood were X-irradiated to obtain doses of 38, 75, 150 or 300 cGy. Controls were sham-irradiated. After exposure to X-rays, mononuclear leukocytes were isolated using density gradients and cultured in RPMI 1640 medium containing phytohemagglutinin to stimulate mitogenesis. At 21 h cytochalasin B was added to produce BN PBLs, and all cultures were harvested at 52 h post-initiation using a cytocentrifuge. Significant dose-dependent increases in the percentage of micronucleated cells and the number of MN per BN cell were observed in all three species. The linear-quadratic regression curves for the total percentage of micronucleated cells for the three species were similar; however, the curve for the mouse PBLs had a larger quadratic component than either of the curves for the rat or human PBLs. Although the correlation between the percentage of cells with MN and those with chromosome aberrations was high (r squared > 0.95), the mouse and rat PBLs were over twice as efficient as human PBLs in forming MN from presumed acentric fragments. These data indicate that the induction of MN in BN cells following ionizing radiation is similar in human, rat and mouse PBLs, but care must be taken in using the MN results to predict frequencies of cells with chromosomal aberrations. (Copyright (c) 1991 Elsevier Science Publishers B.V.)