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Main Title Mutagenic potential of municipal sewage sludge and sludge amended soil /
Author Donnelly, K. C. ; Brown, K. W. ; Chisum, C. P.
Other Authors
Author Title of a Work
Brown, Kenneth W.
Chisum, C. P.
CORP Author Texas A and M Univ., College Station.;Health Effects Research Lab., Research Triangle Park, NC.
Publisher U.S. Environmental Protection Agency, Health Effects Research Laboratory,
Year Published 1987
Report Number EPA/600/D-87/259; EPA-R-812037
Stock Number PB88-103841
Subjects Sewage sludge--Environmental aspects--United States ; Mutagenesis ; Soils--Agricultural chemical content--United States ; Soils--United States--Agricultural chemical content
Additional Subjects Sludge ; Mutagens ; Waste water ; Water treatment ; Microbiology ; Soil science ; Sewage disposal ; Environment effects
Library Call Number Additional Info Location Last
NTIS  PB88-103841 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 32 pages ; 28 cm
Twelve municipal wastewater treatment plant sludges were collected and extracted by sequential extraction with methylene chloride and methanol on a Soxhlet apparatus. Each of three sludge fractions, i.e., methylene chloride, methanol, and a combined fraction (36 fractions total) were tested in the Salmonella/microsome assay. Only one of the sludge fractions induced a positive response in the absence of metabolic activation, and 12 fractions induced a doubling of revertant colonies at two or more consecutive dose levels with activation. The maximum mutagenic response observed with strain TA98 in a methylene chloride fraction was 120 net revertants at a dose level of 10 mg of extract per plate. The maximum response induced by the methanol and combined fractions was 89 net revertants, induced by a municipal sludge with 20% industrial contribution, and 61 net revertants, induced by a municipal sludge with 19% industrial contribution, respectively, both tested with strain TA98 at a dose level of 5 mg of extract per plate. These results indicate that there will be substantial differences in the mutagenic potential of municipal sludges from a single source and from different sources.
Caption title. "Sept. 1987." Includes bibliographical references. "EPA/600/D-87/259." Microfiche.