Record Display for the EPA National Library Catalog


Main Title Susceptibility of Chemostat-Grown 'Yersinia enterocolitica' and 'Klebsiella pneumoniae' to Chlorine Dioxide.
Author Harakeh, M. S. ; Berg, J. D. ; Hoff, J. C. ; Matin, A. ;
CORP Author Stanford Univ., CA. School of Medicine.;Environmental Protection Agency, Cincinnati, OH. Water Engineering Research Lab.
Year Published 1985
Report Number EPA/600/J-85/035;
Stock Number PB85-193704
Additional Subjects Aquatic microbiology ; Bacteria ; Klebsiella ; Chlorine oxides ; Disinfectants ; Resistance ; Reprints ; Enterobacteriacease ; Growth conditions ; Environmental pollution
Library Call Number Additional Info Location Last
NTIS  PB85-193704 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 5p
The resistance of bacteria to antimicrobial agents could be influenced by growth environment. The susceptibility of two enteric bacteria, Yersinia enterocolitica and Klebsiella pneumoniae, to chlorine dioxide was investigated. These organisms were grown in a defined medium in a chemostat and the influence of growth rate, temperature, and cell density on the susceptibility was studied. All inactivation experiments were conducted with a dose of 0.25 mg of chlorine dioxide per liter in phosphate-buffered saline at pH 7.0 and 23C. The results indicated that populations grown under conditions that more closely approximate natural aquatic environments, e.g., low temperatures and growth at submaximal rates caused by nutrient limitation, were most resistant. The conclusion from this study is that antecedent growth conditions have a profound effect on the susceptibility of bacteria to disinfectants, and it is more appropriate to use the chemostat-grown bacteria as test organisms to evaluate the efficacy of a certain disinfectant. (Copyright (c) 1985, American Society for Microbiology.)