| Main Title |
Inhibin Increases and Progesterone Decreases Receptors for Gonadotropin-Releasing Hormone in Ovine Pituitary Culture. |
| Author |
Laws, S. C. ;
Beggs, M. J. ;
Webster, J. C. ;
Miller., W. L. ;
|
| CORP Author |
North Carolina State Univ. at Raleigh. Dept. of Biochemistry.;Health Effects Research Lab., Research Triangle Park, NC. |
| Publisher |
c1990 |
| Year Published |
1990 |
| Report Number |
EPA/600/J-90/178; |
| Stock Number |
PB91-116889 |
| Additional Subjects |
Progesterone ;
Physiology ;
Pituitary gland ;
Sheep ;
In vitro analysis ;
Reprints ;
Gonadotropin receptors ;
Inhibin ;
Cultured cells ;
Competitive binding ;
Dose-response relationships
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB91-116889 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
9p |
| Abstract |
Treatments (48 h) with highly purified bovine or porcine inhibins (10 ng/ml) induced ovine pituitary cells to increase their binding for des-Gly(to the tenth power)-(D-Ala(to the sixth power))LHRH-ethylamide by 3.6- and 5-fold, respectively. Studies with less pure inhibin from porcine follicles showed that increased binding could reach 7-fold within 48 h and was due to higher numbers of receptors for GnRH. The 48-h increase in GnRH receptors was linear with time and was rapidly reversible, since removal of inhibin at 24 h decreased GnRH binding below control levels at 48 h. Inhibin (bovine or porcine) also increased GnRH-stimulated secretion of LH by 2-fold. The ED50 for both inhibin actions noted above was in the range of 0.5-2.0 ng/ml (in terms of highly purified bovine inhibin). Progesterone (P) totally counteracted inhibin induction of GnRH binding and GnRH-stimulated LH secretion at 48 h. (Copyright (c) 1990 by The Endocrine Society.) |
