Record Display for the EPA National Library Catalog


Main Title Microcosm Method to Assess Survival of Recombinant Bacteria Associated with Plants and Herbivorous Insects (Journal Version).
Author Armstrong, J. L. ; Knudsen, G. R. ; Seidler, R. J. ;
CORP Author Corvallis Environmental Research Lab., OR. ;Oregon State Univ., Corvallis. Dept. of Microbiology.
Publisher c1987
Year Published 1987
Report Number EPA/600/J-87/455;
Stock Number PB89-134613
Additional Subjects Genetic intervention ; Recombinant DNA ; Plants(Botany) ; Ecosystems ; Population dynamics ; Survival ; Plasmids ; Reprints ; Pseudomonas cepacia ; Cutworms ; Peridroma saucia
Library Call Number Additional Info Location Last
NTIS  PB89-134613 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 6p
A microcosm method was developed to investigate survival and fate of genetically engineered bacteria associated with plant surfaces and a plant-feeding insect, the variegated cutworm, Peridroma saucia. Larvae on radish plants in microcosms were sprayed with nonrecombinant Pseudomonas cepacia and a recombinant strain of P. cepacia carrying the transmissible plasmid R388::Tn1721. Immediately after spraying, P. cepacia comprised about 20%-30% of the total population on leaves. After 2 days, the total epiphytic population had increased approximately fourfold, while the P. cepacia strains had decreased to 2%-30% of their initial numbers. Plasmid transfer from P. cepacia R388::Tn 1721 to the nonrecombinant recipient P. cepacia strain was not observed. (Copyright (c) Springer-Verlag New York Inc. 1987.)