Record Display for the EPA National Library Catalog


Main Title Simple Mini-Method to Extract DNA Directly from Soil for Use with Polymerase Chain Reaction Amplification.
Author Porteous, L. A. ; Armstrong, J. L. ;
CORP Author Corvallis Environmental Research Lab., OR.
Publisher c1993
Year Published 1993
Report Number EPA/600/J-93/288;
Stock Number PB93-222792
Additional Subjects Deoxyribonucleic acids ; Enzymes ; Soils ; Electrophoresis ; Bacteria ; Fungi ; Extraction ; Separation ; Reprints ; Polymerase chain reactions ; Ecological studies
Library Call Number Additional Info Location Last
NTIS  PB93-222792 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 6p
A rapid, simple method is used that yields amplifiable fungal and bacterial DNAs directly from soil. DNA is separated from soil contaminants by electrophoresis in low-melting-temperature agarose and used directly in polymerase chain reaction amplification. Fifty 20-mg samples can be processed in one day. Fragments of 16S and 18S ribosomal RNAs are amplified by polymerase chain reaction with DNA extracted from the soil. Universal primers are used that are capable of amplifying ribosomal DNA from a wide variety of bacteria and fungi. Eubacterial and fungal primers are used that are capable of distinguishing between eubacterial and fungal DNAs. Restriction enzyme digests are performed on amplified DNA fragments from five soil samples. (Copyright (c) Springer-Verlag Inc. 1993.)