Record Display for the EPA National Library Catalog


Main Title Restriction Fragment Length Polymorphisms Distinguish Ectomycorrhizal Fungi.
Author Armstrong, J. L. ; Fowles, N. L. ; Rygiewicz, P. T. ;
CORP Author Corvallis Environmental Research Lab., OR. ;NSI Technology Services Corp., Corvallis, OR.
Publisher c1989
Year Published 1989
Report Number EPA/600/J-89/112;
Stock Number PB90-108432
Additional Subjects Plant genetics ; Deoxyribonucleic acids ; Taxonomy ; Reprints ; Restriction fragment length polymorphisms ; Ectomycorrhizal fungi ; Nucleic acid hybridization ; DNA probes ; Laccaria bicolor ; Laccaria laccata
Library Call Number Additional Info Location Last
NTIS  PB90-108432 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 9p
Basidiomycetous fungi, two saprophytes and three mycorrhizal, were used to assess the specificity of DNA hybridization for distinguishing genera from one another. Interspecific comparisons were done with several isolates of mycorrhizal fungi, Laccaria bicolor and L. Laccata, collected from diverse geographical sites. The DNAs were digested with four restriction nucleases and separated by gel electrophoresis into patterns of DNA fragments called restriction fragment length polymorphism (RFLPs). The RFLPs were hybridized with a radioactively-labeled DNA probe encoding Basidiomycetous ribosomal RNA genes. The five genera were discernable using both unprobed and probed RFLPs. Hybridization of probe DNA with RFLPs was isolate-specific for all nine Laccaria isolates examined. The reclassification of a L. bicolor isolate is supported, demonstrating that hybridization of RFLPs offers an additional tool for taxonomy of ectomycorrhizal fungi. The method may have field application for distinguishing known isolates if their DNA fingerprints are previously ascertained and are distinct from RFLPs of indigenous organisms. (Copyright (c) Kluwer Academic Publishers 1989.)