Record Display for the EPA National Library Catalog


Main Title Predictive Model of Conjugative Plasmid Transfer in the Rhizosphere and Phyllosphere.
Author Knudsen, G. R. ; Walter, M. V. ; Porteous, L. A. ; Prince, V. J. ; Armstrong, J. L. ;
CORP Author Corvallis Environmental Research Lab., OR.
Publisher 1988
Year Published 1988
Report Number EPA/600/J-88/297;
Stock Number PB89-202329
Additional Subjects Survival ; Computerized simulation ; Reprints ; Plasmids ; Pseudomonas cepacia ; Rhizospheres ; Phyllospheres ; Genetic conjugation
Library Call Number Additional Info Location Last
NTIS  PB89-202329 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 7p
A computer simulation model was used to predict the dynamics of survival and conjugation of Pseudomonas cepacia (carrying the transmissible recombinant plasmid R388:Tn1721) with a nonrecombinant recipient strain in simple rhizosphere and phyllosphere microcosms. Plasmid transfer rates were derived for a mass action model, and donor and recipient survival were modeled as exponential growth and decay processes or both. Rate parameters were derived from laboratory studies in which donor and recipient strains were incubated in test tubes with a peat-vermiculite solution or on excised radish or bean leaves in petri dishes. The model predicted donor, recipient, and transconjugant populations in hourly time steps. It was tested in a microcosm planted with radish seeds and inoculated with donor and recipient strains and on leaf surfaces of radish and bean plants also growing in microcosms. Bacteria were periodically enumerated on selective media over 7 to 14 days. An initial rapid increase and a subsequent decline in numbers of transconjugants in the rhizosphere and on leaf surfaces were correctly predicted.