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Main Title Immunotoxicologic Assessment of Subacute Exposure of Rats to Carbon Tetrachloride with Comparison to Hepatotoxicity and Nephrotoxicity.
Author Smialowicz, R. J. ; Simmons, J. E. ; Luebke, R. W. ; Allis., J. W. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC.
Publisher c1991
Year Published 1991
Report Number EPA/600/J-91/181;
Stock Number PB91-233445
Additional Subjects Carbon tetrachloride ; Liver ; Kidney ; Toxicology ; Immune system ; Rats ; Dose-response relationships ; Antibodies ; Body weight ; Natural killer cells ; Spleen ; Thymus gland ; Cytochrome p-450 ; Alanine aminotransferase ; Aspartate aminotransferase ; Reprints ;
Library Call Number Additional Info Location Last
NTIS  PB91-233445 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 12p
The immunotoxicity, hepatotoxicity, and nephrotoxicity of subacute exposure to carbon tetrachloride (CCl4) were evaluated in young adult (8-9 weeks old) male Fischer 344 rats dosed by gavage with CCl4 for 10 consecutive days at 0, 5, 10, 20 or 40 mg/kg/day. Two days following the last treatment rats were evaluated for alterations in immune function by monitoring the following: body and lymphoid organ weights; mitogen and mixed leukocyte reaction lymphoproliferative responses; natural killer cell activity; and cytotoxic T lymphocyte responses. A separate group of similarly dosed rats was immunized with sheep red blood cells (SRBC) on Day 9 of dosing, and the primary antibody response was assessed 4 days later. Hepatic and renal toxicity were assessed 2 days after the last treatment by monitoring organ weights, serum indicators of hepatic and renal damage, and hepatic cytochrome P450 levels, as well as by histological evaluation. Significant increases in relative liver weights were observed in rats dosed at 40 mg/kg/day. Histologically, these livers displayed mild to moderate vacuolar degeneration and minimal to mild hepatocellular necrosis. In addition, serum levels of aspartate aminotransferase and alanine aminotransferase were elevated at this dosage, as well as at 20 mg/kg/day. (Copyright (c) 1991 by the Society of Toxicology.)