||Kinetochore-Staining of Spermatid Micronuclei: Studies of Mice Treated with X-Radiation or Acrylamide.
Collins, B. W. ;
Howard, D. R. ;
Allen, J. W. ;
||Health Effects Research Lab., Research Triangle Park, NC. Genetic Toxicology Div. ;Environmental Health Research and Testing, Inc., Research Triangle Park, NC.
X rays ;
Fluorescent antibody technique ;
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
The rodent spermatid micronucleus (MN) assay was used in conjunction with immunofluorescent techniques to distinguish kinetochores in MN following exposure of mice to X-radiation or acrylamide. After either treatment, modest increases in kinetochore-positive MN were observed. Spermatids which had been exposed during meiotic prophase to X-rays (400 cGy) had approximately 10-fold increases in MN compared to controls; up to 15% of the MN observed were kinetochore-positive. Following acrylamide treatment of meiotic prophase cells, there was a doubling of spermatid MN over baseline levels, approximately one-third of which were kinetochore-positive. (Copyright (c) 1992 Elsevier Science Publishers B.V.)