||Environmental technology verification report : PCB detection technology : Hybrizyme Delfia (trademart) PCB assay /
Dindal, Amy B. ;
Bayne, C. K. ;
Jenkins, R. A. ;
Koglin, E. N.
||Oak Ridge National Lab., TN.;Environmental Protection Agency, Las Vegas, NV. National Exposure Research Lab.
|| U.S. Environmental Protection Agency, Office of Research and Development, National Exposure Research Laboratory, Environmental Sciences Division,
Polychlorinated biphenyls ;
Technology verification ;
Analytical techniques ;
Solvent extracts ;
Logistical operations ;
Verification tests ;
Laboratory analyses ;
Immunoassay kits ;
Environmental Protection Agency ;
DELFIA PCB Assay ;
Monoclonal antibody reagents
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
||50 pages : illustrations ; 28 cm
The DELFIA PCB Assay is a solid-phase time-resolved fluoroimmunoassay based on the sequential addition of sample extract and europium-labeled PCB tracer to a monoclonal antibody reagent specific for PCBS. In this assay, the antibody reagent and sample extract are added to a strip of Microtiter plate wells and allowed to react. The strips have been specially treated to trap the antibody reagent or antibody-PCB complexes that may have formed. A wash step removes sample matrix from the captured antibody. This step significantly reduces any potential matrix interferences before the addition of the PCB tracer, resulting in an unusually robust assay system. The PCB tracer is then added and allowed to bind the antibodies that are not complexed with sample PCBS. A wash step is used to separate antibody-bound tracer from the tracer free in solution. The addition of an enhancement solution forms highly fluorescent chelates with the bound europium ions. The amount of fluorescence measured is inversely proportional to the concentration of PCBs in the sample. The lower reporting level is typically 0.5 ppm.
Cover title. "August 2001." "EPA/600/R-01/052." Microfiche.