Grantee Research Project Results
Publications Details for Grant Number R825354
Microbial Monitoring With Artificial Stable RNAs
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Reference Type | Reference Title | Journal | Author | Citation | Progress Report Year | Document Sources |
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Abstract | Environmental monitoring of microorganisms using artificial rRNA label and molecular beacons. | ENVIRONMENTAL SCIENCE & TECHNOLOGY | Kourentzi KD, Walia RP, Murphy JC, Wibbenmeyer J, Larios-Sanz M, Fox GE, Willson RC | Kourentzi KD, Walia RP, Murphy JC, Wibbenmeyer J, Larios-Sanz M, Fox GE, Willson RC. Environmental monitoring of microorganisms using artificial rRNA label and molecular beacons. Abstracts of Papers of the American Chemical Society 1999;217(BIOT Pt 1):U179-U180. |
R825354 (Final) |
not available |
Book Chapter | Estadio de las poblaciones microbiologicas en el ambiente espacial (Molecular tools for monitoring microbial populations in space environments). | None | Larios-Sanz M, Kourentzi KD, Murphy JC, Maillard KI, Pearson DL, Willson RC, Fox GE | Larios-Sanz M, Kourentzi KD, Murphy JC, Maillard KI, Pearson DL, Willson RC, Fox GE. Estadio de las poblaciones microbiologicas en el ambiente espacial (Study of the microbiological populations in the space atmosphere). In: Mas-Oliva J, Soriano CAM, eds. Diagnostico Molecular en Medicina. Mexico City, Mexico: Manual Moderno, 2004, Chapter 13, pp. 293-311. |
R825354 (Final) |
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Dissertation/Thesis | Development of an expression system to study 5S rRNA mutants in P. putida and its application as a tool for microbial monitoring. | None | D'Souza LM | D'Souza LM. Development of an expression system to study 5S rRNA mutants in P. putida and its application as a tool for microbial monitoring. Ph.D. Dissertation, Department of Biology and Biochemistry, University of Houston, Houston, TX, December 1999. |
R825354 (Final) |
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Dissertation/Thesis | Environmental monitoring of microorganisms using 5S rRNA and molecular beacons. | None | Kourentzi ED | Kourentzi ED. Environmental monitoring of microorganisms using 5S rRNA and molecular beacons. M.S. Thesis, Department of Chemical Engineering, University of Houston, Houston, TX, 1999. |
R825354 (1999) R825354 (Final) |
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Dissertation/Thesis | Studies in nucleic acid separations. | None | Murphy JC | Murphy JC. Studies in nucleic acid separations. Ph.D. Thesis, Department of Chemical Engineering, University of Houston, Houston, TX, December 2000. |
R825354 (Final) |
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Dissertation/Thesis | Microbial monitoring using hybridization assays and artificial RNA labels. | None | Walia RP | Walia RP. Microbial monitoring using hybridization assays and artificial RNA labels. M.S. Thesis, Department of Biomedical Engineering, University of Houston, Houston, TX, January 1998. |
R825354 (Final) |
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Journal Article | A genomically modified marker strain of Escherichia coli. | CURRENT MICROBIOLOGY | Ammons D, Rampersad J, Fox GE | Ammons D, Rampersad J, Fox GE. A genomically modified marker strain of Escherichia coli. Current Microbiology 1998;37(5):341-346. |
R825354 (1999) R825354 (Final) |
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Journal Article | 5S rRNA gene deletions cause an unexpectedly high fitness loss in Escherichia coli. | NUCLEIC ACIDS RESEARCH | Ammons D, Rampersad J, Fox GE | Ammons D, Rampersad J, Fox GE. 5S rRNA gene deletions cause an unexpectedly high fitness loss in Escherichia coli. Nucleic Acids Research 1999;27(2):637-642. |
R825354 (1999) R825354 (Final) |
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Journal Article | Expression of marker RNAs in Pseudomonas putida. | CURRENT MICROBIOLOGY | D'Souza LM, Willson RC, Fox GE | D'Souza LM, Willson RC, Fox GE. Expression of marker RNAs in Pseudomonas putida. Current Microbiology 2000;40(2):91-95. |
R825354 (1999) R825354 (Final) |
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Journal Article | Purification of plasmid DNA using selective precipitation by compaction agents. | NATURE BIOTECHNOLOGY | Murphy JC, Wibbenmeyer JA, Fox GE, Willson RC | Murphy JC, Wibbenmeyer JA, Fox GE, Willson RC. Purification of plasmid DNA using selective precipitation by compaction agents. Nature Biotechnology 1999;17(8):822-823. |
R825354 (1999) R825354 (Final) |
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Journal Article | RNA isolation and fractionation with compaction agents. | NUCLEIC ACIDS RESEARCH | Murphy JC, Fox GE, Willson RC | Murphy JC, Fox GE, Willson RC. RNA isolation and fractionation with compaction agents. Analytical Biochemistry 2001;295(2):143-148. |
R825354 (Final) |
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Journal Article | Enhancement of anion-exchange chromatography of DNA using compaction agents. | None | Murphy JC, Fox GE, Willson RC | Murphy JC, Fox GE, Willson RC. Enhancement of anion-exchange chromatography of DNA using compaction agents. Journal of Chromatography A 2003;984(2):215-221. |
R825354 (Final) |
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Journal Article | Nucleic acid separations utilizing immobilized metal affinity chromatography. | None | Murphy JC, Jewell DL, White KI, Fox GE, Willson RC | Murphy JC, Jewell DL, White KI, Fox GE, Willson RC. Nucleic acid separations utilizing immobilized metal affinity chromatography. Biotechnology Progress 2003;19(3):982-986. |
R825354 (Final) |
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Journal Article | A low molecular weight artificial RNA of unique size with multiple probe target regions. | SYSTEMATIC AND APPLIED MICROBIOLOGY | Pitulle C, Dsouza L, Fox GE | Pitulle C, Dsouza L, Fox GE. A low molecular weight artificial RNA of unique size with multiple probe target regions. Systematic and Applied Microbiology 1997;20(1):133-136. |
R825354 (1997) R825354 (Final) |
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Journal Article | Boronate affinity adsorption of RNA: possible role of conformational changes. | JOURNAL OF CHROMATOGRAPHY A | Singh N, Willson RC | Singh N, Willson RC. Boronate affinity adsorption of RNA: possible role of conformational changes. Journal of Chromatography A 1999;840(2):205-213. |
R825354 (1997) R825354 (1999) R825354 (Final) |
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Meeting | Texas American Society of Microbiology Branch Meeting. | None | George E. Fox | Invited Symposium Speaker, Texas American Society of Microbiology Branch Meeting, Houston, Texas, November 6-7, 1997. |
R825354 (1997) |
not available |
Patent/Patent Application | Apparatus, methods and compositions for biotechnical separations. | None | Willson III RC, Murphy JC | Willson III RC, Murphy JC. Apparatus, methods and compositions for biotechnical separations. U.S. Patent Application No. US 09/841,763, January 24, 2002. |
R825354 (Final) |
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Presentation | 5S rRNA as a tool for studying molecular evolution. | None | DSouza, L. M., Lee, Y-H., Pitulle, C., Fox, G. E. | DSouza, L. M., Lee, Y-H., Pitulle, C., and Fox, G. E.; "5S rRNA as a tool for studying molecular evolution" Abstracts- RNA '97, p212, Banff, Alberta, Canada , May 27-June 1, 1997. |
R825354 (1997) |
not available |
Presentation | Experimental characterization of the sequence space in the vicinity of Vibrio proteolyticus 5S rRNA. | None | Fox GE | Fox GE. Experimental characterization of the sequence space in the vicinity of Vibrio proteolyticus 5S rRNA. Presented at the University of Maine, Orono, ME, October 15, 1999. |
R825354 (1999) R825354 (Final) |
not available |
Presentation | Tracking E. coli: Artificial Stable RNAs in Bacterial Monitoring. | None | George E. Fox | Invited Sigma Chi Lecture, "Tracking E. coli: Artificial Stable RNAs in Bacterial Monitoring", Centers for Disease Control, Atlanta, Ga., October 30, 1997. |
R825354 (1997) |
not available |
Presentation | Environmental monitoring of microorganisms using artificial rRNA labels and molecular beacons. | None | Kourentzi KD, Walia RP, Murphy JC, Wibbenmeyer JA, Larios-Sanz M, Fox GE, Willson RC | Kourentzi KD, Walia RP, Murphy JC, Wibbenmeyer JA, Larios-Sanz M, Fox GE, Willson RC. Environmental monitoring of microorganisms using artificial rRNA labels and molecular beacons. Presented at the 17th Annual Houston Conference on Biomedical Engineering, Houston, TX, February 11-12, 1999. |
R825354 (1999) R825354 (Final) |
not available |
Presentation | Environmental monitoring of microorganisms using artificial rRNA labels and molecular beacons. | None | Kourentzi KD, Walia RP, Murphy JC, Wibbenmeyer JA, Larios-Sanz M, Fox GE, Willson RC | Kourentzi KD, Walia RP, Murphy JC, Wibbenmeyer JA, Larios-Sanz M, Fox GE, Willson RC. Environmental monitoring of microorganisms using artificial rRNA labels and molecular beacons. Presented at the 217th National Meeting of the American Chemical Society, Anaheim, CA, March 21-25, 1999, Abstract No. BIOT-088. |
R825354 (1999) R825354 (Final) |
not available |
Presentation | Monitoring microorganisms using hybridization assays specific for rRNA. | None | Kourentzi KD, Fox GE, Willson RC | Kourentzi KD, Fox GE, Willson RC. Monitoring microorganisms using hybridization assays specific for rRNA. Presented at the 219th National Meeting of the American Chemical Society, San Francisco, CA, March 26-30, 2000, Abstract No. BIOT-279. |
R825354 (Final) |
not available |
Presentation | Environmental monitoring using rRNA labels and hybridization assays. | None | Kourentzi K, Larios-Sanz M, Setterquist RA, Fox GE, Willson RC | Kourentzi K, Larios-Sanz M, Setterquist RA, Fox GE, Willson RC. Environmental monitoring using rRNA labels and hybridization assays. Presented at the Gordon Conference on Applied & Environmental Biology, Connecticut College, New London, CT, July 3-8, 1999. |
R825354 (1999) R825354 (Final) |
not available |
Presentation | Small RNA sequences are readily stabilized by inclusion in a carrier rRNA. | None | Larkin D, Larios-Sanz M, Setterquist RA, D'Souza LD, Willson RC, Fox GE | Larkin D, Larios-Sanz M, Setterquist RA, D'Souza LD, Willson RC, Fox GE. Small RNA sequences are readily stabilized by inclusion in a carrier rRNA. Presented at RNA '99, the 4th Annual Meeting of the RNA Society, University of Edinburgh, Scotland, UK, June 23-27, 1999. |
R825354 (1999) R825354 (Final) |
not available |
Presentation | Nucleic acids separation using compaction agents. | None | Murphy JC, Fox GE, Willson RW | Murphy JC, Fox GE, Willson RW. Nucleic acids separation using compaction agents. Presented at the 218th National Meeting of the American Chemical Society, Boston, MA, August 26, 1999. |
R825354 (Final) |
not available |
Presentation | Separation of nucleic acids using compaction agents. | None | Murphy JC, Fox GE, Willson RC | Murphy JC, Fox GE, Willson RC. Separation of nucleic acids using compaction agents. Presented at the 13th International Symposium on Affinity Technology and Bio-Recognition, Compiegne, France, July 8, 1999 (invited). |
R825354 (Final) |
not available |
Presentation | Nucleic acids separations using compaction agents. | None | Murphy JC, Wibbenmeyer JA, Fox GE, Willson RC | Murphy JC, Wibbenmeyer JA, Fox GE, Willson RC. Nucleic acids separations using compaction agents. Presented at the American Institute of Chemical Engineers 1999 Spring Meeting, Houston, TX, March 14-18, 1999. |
R825354 (1999) R825354 (Final) |
not available |
Presentation | RNA separation using compaction precipitation. | None | Murphy JC, Fox GE, Willson RC | Murphy JC, Fox GE, Willson RC. RNA separation using compaction precipitation. Presented at the 217th National Meeting of the American Chemical Society, Anaheim, CA, March 21-25, 1999. |
R825354 (1999) R825354 (Final) |
not available |
Presentation | New approaches to nucleic acid separation. | None | Murphy JC, White KI, Fox GE, Willson RW | Murphy JC, White KI, Fox GE, Willson RW. New approaches to nucleic acid separation. Presented at the Bioseparations Center, University BOKU, Vienna, Austria, February 2000 (invited). |
R825354 (Final) |
not available |
Presentation | Nucleic acid separation using compaction agents. | None | Murphy JC, White KI, Fox GE, Willson RC | Murphy JC, White KI, Fox GE, Willson RC. Nucleic acid separation using compaction agents. Presented at the 219th National Meeting of the American Chemical Society, San Francisco, CA, March 26-30, 2000, Abstract No. BIOT-205. |
R825354 (Final) |
not available |
Presentation | New approaches to nucleic acid separation. | None | Murphy JC, White KI, Fox GE, Willson RW | Murphy JC, White KI, Fox GE, Willson RW. New approaches to nucleic acid separation. Presented at the 20th International Symposium on the Separation and Analysis of Proteins, Peptides, and Polynucleotides, Ljubljana, Slovenia, November 5-8, 2000 (invited). |
R825354 (Final) |
not available |
Presentation | Separation of nucleic acids using immobilized metal affinity. | None | Murphy JC, Fox GE, Willson RC | Murphy JC, Fox GE, Willson RC. Separation of nucleic acids using immobilized metal affinity. Presented at the 14th International Symposium on Affinity Technology and Recognition, San Carlos, Mexico, April 29-May 2, 2001 (invited). |
R825354 (Final) |
not available |
Presentation | 1998 Fall National Meeting, American Chemical Society. | None | R. C. Willson | 1998 Fall National Meeting, American Chemical Society, Division of Biochemical Technology. |
R825354 (1997) |
not available |
Presentation | 1998 Ibero-American Conference on Biochemical Technology, Lisbon, Portugal. | None | R. C. Willson | 1998 Ibero-American Conference on Biochemical Technology, Lisbon, Portugal, June, 1998. |
R825354 (1997) |
not available |
Presentation | Microbial monitoring with artificial stable RNAs | None | Willson RC | Willson RC. Microbial monitoring with artificial stable RNAs. Presented at the 18th Eighth International Symposium on the Separation and Analysis of Proteins, Peptides and Polynucleotides, Vienna, Austria, November 1-4, 1998. |
R825354 (Final) |
not available |
Presentation | Technologies for nucleic acid hybridization assays. | None | Willson RC | Willson RC. Technologies for nucleic acid hybridization assays. Presented at the Department of Chemical Engineering, University of Wisconsin, Madison, WI, February 1999. |
R825354 (Final) |
not available |
Presentation | Recognition and separation of RNA and DNA. | None | Willson RC | Willson RC. Recognition and separation of RNA and DNA. Presented at the 13th International Symposium on Affinity Technology and Bio-recognition, Compiegne, France, July 4-8, 1999. |
R825354 (1999) R825354 (Final) |
not available |
Presentation | Compaction precipitation for nucleic acid purification. | None | Willson RC | Willson RC. Compaction precipitation for nucleic acid purification. Presented at Recovery of Biological Products IX, Whistler, British Columbia, Canada, May 23-28, 1999. |
R825354 (1999) R825354 (Final) |
not available |
Proceedings | Advanced approach to simultaneous monitoring of multiple bacteria in space. | None | Eggers M, Balch W, Mendoza L, Gangadharan R, Mallik A, McMahon M, Hogan M, Powdrill T, Iverson B, Fox G, Willson RC, Maillard K, Siefert J, Singh N | Eggers M, Balch W, Mendoza L, Gangadharan R, Mallik A, McMahon M, Hogan M, Powdrill T, Iverson B, Fox G, Willson RC, Maillard K, Siefert J, Singh N. Advanced approach to simultaneous monitoring of multiple bacteria in space. In: Proceedings of the 27th International Conference on Environmental Systems, July 1997, Paper No. 972422. |
R825354 (1997) R825354 (Final) |
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Proceedings | Technologies for nucleic acid hybridization assays. | None | Willson, R. C. | Willson, R. C. "Technologies for nucleic acid hybridization assays", Department of Chemical Engineering, University of Wisconsin, Madison, Wisconsin, February, 1999. |
R825354 (1999) |
not available |
Symposium | Eighth International Symposium on Analysis and Separation of Proteins, Peptides and Polynucleotides. | None | R. C. Willson | Eighth International Symposium on Analysis and Separation of Proteins, Peptides and Polynucleotides, Vienna, November, 1998. |
R825354 (1997) R825354 (1999) |
not available |
Symposium | Adsorption/Desorption Processes and Related Separation Techniques. | None | Willson RC | 1998 International Symposium on Ion Exchange, Adsorption/Desorption Processes and Related Separation Techniques, Washington, DC, June, 1998. |
R825354 (1997) |
not available |
The perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Conclusions drawn by the principal investigators have not been reviewed by the Agency.