Grantee Research Project Results
Molecular detection of viable bacterial pathogens in water by ratiometric pre-rRNA analysis.
Citation:
Cangelosi GA, Weigel KM, Lefthand-Begay C, Meschke JS. Molecular detection of viable bacterial pathogens in water by ratiometric pre-rRNA analysis. Applied and Environmental Microbiology 2010;76(3):960-962.
Abstract:
Ratiometric pre-rRNA analysis (RPA) detects the replenishment of rRNA precursors that occurs rapidly upon nutritional stimulation of bacterial cells. In contrast to DNA detection by PCR, RPA distinguishes viable from inactivated bacteria. It exhibits promise as a molecular viability test for pathogens in water and other environmental samples.The perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Conclusions drawn by the principal investigators have not been reviewed by the Agency.