Grantee Research Project Results
Functionalized glass substrate for microarray analysis.
Citation:
DeRosa RL, Cardinale JA, Cooper A. Functionalized glass substrate for microarray analysis. Thin Solid Films 2007;515(7-8):4024-4031.
Abstract:
A series of glass treatments were investigated to develop a miniaturized enzyme-linked immunosorbent assay microarray sensor with covalently immobilized antibody. Two glass compositions were photo-hydrolytically treated and functionalized with an amino- or mercapto-silane. Surface characterization was done using contact angle, X-ray photoelectron spectroscopy and atomic force microscopy data. Antibody binding efficiency was statistically compared between treatments and glass compositions.
Photo-hydrolytic treatment increased surface roughness and hydrophobic behavior due to alkali leaching and molecular surface rearrangement. Antibody binding tests indicated amino functionalized surfaces coupled with photo-hydrolytic treatment adsorb more antibody than those with thiol functionalization and without photo-hydrolytic treatment.
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