Grantee Research Project Results
Final Report: Center for Children's Health, the Environment, Microbiome, and Metabolomics
EPA Grant Number: R836153Center: Center for Children’s Health, the Environment, Microbiome, and Metabolomics’ Center
Center Director: McCauley, Linda
Title: Center for Children's Health, the Environment, Microbiome, and Metabolomics
Investigators: McCauley, Linda , Ryan, P. Barry
Institution: Emory University
EPA Project Officer: Callan, Richard
Project Period: September 1, 2015 through August 31, 2019 (Extended to August 31, 2020)
Project Amount: $1,797,870
RFA: Children's Environmental Health and Disease Prevention Research Centers (2014) RFA Text | Recipients Lists
Research Category: Human Health , Children's Health
Objective:
Project 1:
Aim 1: Characterize pre- and postnatal environmental exposures of African American (AA) mother-infant pairs in metropolitan Atlanta.
Aim 2: Investigate the independent and interactive effects of prenatal chemical exposures upon the composition of the maternal microbiome for pregnant AA women in metropolitan Atlanta. Aim 3: Investigate the independent and interactive effects of prenatal chemical exposures upon birth outcomes for pregnant AA women in metropolitan Atlanta.
Project 2:
Aim 1: Characterize the relationship between prenatal and postnatal environmental exposures and the infant gut microbiome across the first year of life.
Aim 2: Evaluate the associations among prenatal and postnatal toxicant exposures, the infant gut microbiome, maternal caregiving, and infant neurocognitive and social-emotional development.
Project 3:
Aim 1: Identify maternal prenatal metabolic pathways and metabolite-microbiome correlations that link environmental exposures and preterm birth.
Aim 2: Identify maternal prenatal metabolic pathways and metabolite-microbiome correlations that link environmental exposures and infant neurodevelopment.
Aim 3: Identify infant postnatal metabolic pathways and metabolite-microbiome correlations that link environmental exposures and infant neurodevelopment.
Community Outreach and Translation
- Maintain and expand bi-directional dialogue with metropolitan African American (AA) women of child- bearing age and their families
- Develop strategies to translate existing children’s environmental health knowledge and emerging findings into practical information that families can use to protect their children’s health.
- Guide C-CHEM2 scientists in community engagement and outreach.
- Integrate existing children’s environmental health knowledge and new C-CHEM research findings into educational programs for healthcare professionals.
Summary/Accomplishments (Outputs/Outcomes):
Project 1:
Enrollment, Data Collection: We have continued to implement all protocols and procedures for the recruitment and consent of subjects, the collection of data and specimens
(questionnaire items, biological and household samples), and the handling, processing, and analysis of specimens that were developed and refined by the P1 Team during Year 1. During this Year, we maintained Emory Institutional Review Board approval for the P1 recruitment and data collection protocols; utilized data bases for participant and sample tracking; and maintained training of field staff in the consent, data collection, data and sample handling and processing procedures. During March 2020 through July 2020, we did experience delays related to the COVID-19 pandemic in that we were not able to
recruit/enroll new participants or collect data from previously enrolled participants during the
20- 30 week home collection visit window or the 24-30 week prenatal clinic visit 2 collection window; also during this period, we were not able to conduct assays within the university laboratory.
Prenatal: We have enrolled 403 pregnant women into the P50 protocol. Among the 403 enrolled pregnant women, the following completed the planned sample collections during the initial prenatal clinic visit (between 8-14 weeks’ gestation): 396 completed microbiome swab collections, 393 completed venous blood collection, and 393 completed urine sample collection. Of the 403 enrolled, 189 (47%) consented to participate and completing the home assessments, including environmental questionnaires, home urine collection, and home dust sampling. Of the 403 enrolled, 238 (59%) completed the planned sample collections during the second prenatal clinic visit (between 24-30 weeks’ gestation): 238 completed microbiome swab collections, 238 completed venous blood collection, and 229 completed urine sample collection. All biological and environmental specimens from enrolled subjects have been processed and entered into a long-term biorepository created for this project. We have maintained participant tracking in a Microsoft Access database and participant survey and assay data in a RedCap database.
Postnatal: Consistent with our developed protocols and procedures, we also nitiated recruitment of birthed infants into P1 in December 2015. To date, we have collected the following number of urine samples from diapers at the various post-birth time points: 70 samples at 1-week, 49 samples at 3-months, 34 samples at 6-months, and 24 samples at 12-months, and 13 samples at 18-months.
Exposure and Outcome Characterization
Environmental Toxicants:
We performed pilot testing of our laboratory methods for analysis of serum toxicants using serum samples collected from women in the same Parent Study Cohort, under a pilot funding award: Serum samples from the first 184 pregnant African American women enrolled in the Prenatal Microbiome Study were analyzed in the Health and Exposome Research Center: Understanding Lifetime Exposures (HERCULES) Analytic Chemistry Core Lab (the Laboratory for Exposure Assessment and Development in Environmental Research (LEADER) directed by Drs. Barr and Ryan) with funds from a HERCULES pilot award made to a junior faculty mentored by PIs Dunlop and Corwin. This chemical toxicant analysis involves a solvent extraction and cleanup with analysis by gas chromatography-tandem mass spectrometry
In addition, we have performed quantification of urine phthalates, alkylphenols including bisphenol A (BPA), cotinine, THC Acid (marijuana active ingredient metabolite) and current-use pesticides among 390 enrolled pregnant women including N=390 hospital visit 1 samples, N=187 home visit samples and N=228 hospital visit 2 samples for a total of N=805 samples. We found that our population has higher levels of a metabolite of diethylphthalate than does the US population as a whole and US non-Hispanic Black population. Urinary cotinine levels indicate about 30% of our population is actively smoking with is greater than the percentage reporting smoking. Those who had higher THC acid levels were likley to have high cotinine levels as well indicating a combined exposure to tobacco and marijuana. Pesticide metabolite levels are much higher than the most recent NHANES levels which are more than 15 years old indicating pervasive exposure to pyrethroids and OP pesticides in our population.
We measured persistent organic pollutants including DDT, its degradate DDE, PCBs and PBDEs in 482 women. We found higher levels of PBDE congener 47 in our population than in the general US population and DDE levels that were more similar to population-based levels derived over 15 years ago indicating persistently high body burdens of DDE in our population.
We measured poly- and per-fluorinated chemicals in 453 women. We found higher levels of perfluorohexane sulfonic acid in our population as compared to NHANES and a relevant association with Vitamin D levels.
We also measured parabens, bisphenol S, PAHs, heavy metals and metalloids in N=56 women (hospital visit 1 samples) to determine prevalence and magnitude of exposures in our population. Our data indicate widespread exposure to all of these chemicals.
Microbiome:
We have transferred the microbiome swab samples collected on enrolled women to the Emory Integrated Genomics Core for DNA extraction and 16S rRNA gene sequencing; we have established the bioinformatics pipeline to process the raw sequence data using SILVA and PECAN classifiers, which provide an enhanced classification of vaginal-specific microbial genera and species aligning to the V3-V4 primer region
We are presently analyzing the microbiome data to discern exposures that associate with the vaginal microbiome and vaginal microbiome communities that associate with adverse birth outcomes, including preterm birth.
- Pregnancy Outcomes: We have ascertained pregnancy outcomes (via maternal and infant medical record abstraction) for the 403 enrolled women: 41 experienced an early pregnancy loss/spontaneous abortion, 47 experienced a preterm birth, 104 experienced an early term birth, 194 experienced a full term birth, 15 were lost to follow-up (with birth outcome unable to be ascertained as of this time) and 4 remain pregnant.
- Manuscript Preparation: Based on our data collected and analyzed to date, we are preparing the following manuscripts:
- Characterizing exposure in an Atlanta-area African America birth cohort – draft circulating
- Concentrations of Urinary Metabolites of Phthalates in an Atlanta-area African American Birth Cohort;
- Concentrations of Serum PBDE’s in an Atlanta-area African American Birth Cohort
- Concentrations of Alkylated Environmental Phenols in an Atlanta-area African American Birth Cohort
- Concentrations of Urinary Metals in an Atlanta-area African American Birth Cohort
To date, data collected from our study have supported six MPH theses:
- Feminine Hygiene products: A possible source of exposure among pregnant African American women in Atlanta, GA.
- An Analysis of the Effects of Marijuana Use upon Maternal Phthalate Exposure.
- Predictors of PBDE exposure in an Atlanta-area birth cohort: The CHERUB study.
- Variability in Phthalate Measures and Gestational Cardiometabolic Disease in Atlanta African American Mothers.
- Characterization of Tobacco Smoke Exposure and Associated Factors in an African American Birth Cohort.
- Urinary Bisphenol A Levels Among SES-Diverse Pregnant African American Women in Atlanta.
We have interfaced with representatives from the COTC and the Community Advisory Board to discuss strategies for displaying individual- and group-level data and providing feedback about environmental exposures to participants and community members.
Project 1 faculty, staff, and trainees presented their work at a number of local, national, and international conferences. In total they presented 14 podium presentations, two webinars, and four poster presentations. Also, Co-Project Lead Dunlop has participated in two Stakeholder Advisory Board meetings during which updates on the progress-to-date of the Emory Children’s Study Center were shared with the community stakeholders and input was collected from the community stakeholders regarding possible ways for sharing research findings with African American communities, with a focus on women of reproductive age, as well as with the clinical providers who care for these communities.
Project 2:
We have accomplished the following, consistent with our original timeline:
- Data collection for the Microbiome, Environment, and Neurodevelopmental Delay study has continued throughout the length of the project, and we now have 200 mother-infant pairs enrolled in the postnatal follow up;
- We have maintained strong relationships with research participants and this has resulted in a successful retention rate of over 80%. This required significant effort on behalf not only the project leadership but also project staff;
- We have successfully completed assays on the infant microbiome and analyses are continuing at the time of this report;
- We have presented research findings linking cotinine and marijuana exposures to infant neurodevelopmental outcomes as well as fMRI measures (manuscripts under preparation);
- We have two novel manuscripts, one published and one currently under revision linking maternal stress to neurodevelopmental outcomes;
- We have developed and submitted a new P50 proposal focused on environmental exposures and epigenetics and designed to increase diversity in environmental science (score 33; unfunded).
Project 3:
This project was developed as a collaborative project between Dr Elizabeth Corwin, Dr Shuzhao Li and Dean Jones and near the end of the award, Dr Corwin accepted a position at Columbia University and Dr Li accepted a position at Jackson Laboratory. Thus, most of the accomplishments during the total award period involve all three investigators, with Dr Jones maintaining responsibility at the end of the award. Throughout the period of the award, Dr Corwin, Dr Li and Dr Jones collaborated with scientists on Projects 1 and 2 especially toward obtaining a broader understanding of the metabolic pathways associated with environmental exposures and chronic stress.
The team each contributed in different ways to advance the goals of the project. Dr Corwin is a world leader in nursing research and provided a leadership role in bringing together the team to study metabolite, microbiome and environmental interactions impact infant neurodevelopment and birth outcomes. She led research efforts to coordinate sample collection and analyses.
The rapidly changing technology in microbiome research, as well as an important recognition of complexity in environmental effects on microbiome which could not be simply separated from effects of either on metabolomics left the project largely underfunding to fully realize the potential for studying metabolic, microbiome and toxicant-related interactions. The efforts were very fruitful, however, in improving the experimental design and also in prompting a better focus on metabolomics and associations with chronic stress, creating a foundation for more in depth studies of the critical toxicant-related impacts that are mediated through interactions with the microbiome and metabolism
Dr Li is a world authority in systems biology and contributed many new methods papers to promote development of the integrative metabolomics methods needed for the future to understand metabolic, microbiome and toxicant-related interactions. During this award, he developed and published an important state-of-the-art compendium of methods critical for the future to understand environmental effects on health outcomes in pregnancy. Although much of this was funded by other funding sources, the collaborative interaction with this project was key
to promoting this development. His leadership in communicating these interactive environment- metabolome-outcome studies has helped a new generation of scientists understand the critical need for big data approaches to address the poor health associated with environmental chemical exposures, chronic stress and health outcomes in pregnancy. He has also published important environmental exposure and health outcomes in pregnancy that were enabled by the current funding but primarily funded by other sources. Thus, another benefit of the current project was that if facilitated collaboration of a productive research team to advance methodology and knowledge of metabolomics, environmental exposures and health outcomes.
Dr. Jones is a leader in metabolomics and the exposome, with experience in microbiome- related metabolomics as well and environmental chemical exposures and associated metabolic consequences and health outcome. As detailed in the publications cited, important metabolic changes are found in association with environmental exposures and chronic stress in pregnancy. As a consequence of this project, he has specifically developed efforts to understand the components of the metabolome derived from the microbiome and also the specific low-level signals that represent uncharacterized environmental exposures. The currently developing understanding of metabolic change with disease is that most early changes are adaptive in nature. Specifically, the body responds to environmental chemical exposures and chronic stress with metabolic adjustments to protect against adverse effects. Many changes are seen in lipid signaling, inflammation and oxidative stress pathways, which result in reprogramming of mitochondrial metabolism, amino acid metabolism and complex lipid metabolism.
Specifics of some of these responses were observed in metabolomic analyses on the serum samples collected as part of Project 1. Specifically, to characterize the intrauterine environment of this cohort, the laboratory assayed the serum metabolome of the first 320 pregnant African women enrolled with high-resolution liquid chromatography-mass spectroscopy/mass- spectroscopy in the Emory CHEAR Untargeted Analytic Chemistry facility.
To date, the team completed the following analyses adjusting for maternal age, body mass index, and parity:
- Prenatal Metabolism and BDE-47.
- Prenatal Metabolism and Chronic Stress.
- Prenatal Metabolism and Preeclampsia
- Prenatal Metabolism and Preterm Birth
Community Outreach and Translation
The overall objective of the C-CHEM2 SAB was to steer COTC strategy and information sharing in ways that are accessible and culturally relevant to our community partners and intended audiences. The SAB met regularly with Center staff and faculty to provide guidance on assets created by the Center, community concerns, and funding priorities.
The SAB had many independent successes. One board member was selected for the Robert Wood Johnson Foundation Interdisciplinary Research Leaders 2020-2023 cohort. The primary research question her team is addressing is whether geographically focused health messages developed by lay citizens could increase radon testing in communities of color. They will conduct a participatory program-Radon Evaluation, Mitigation, and Ventilation (REMOVE) to empower minority homeowners so that they can effectively take actions to reduce exposures.
Partner Outreach Activities
To foster a meaningful relationship with our SAB members and community, COTC staff maintained regular communication with SAB members, attended partner’s events, and seek out collaborative opportunities with community partners. Examples of community partnerships cultivated and maintained include: Sankofa Cultural Breastfeeding Coalition, Healthy Mothers Health Babies Coalition of Georgia Policy Task Force, First Steps GA, Parents as Teachers of Georgia, Sheltering Arms, BLKHLTH, Centering Pregnancy at Grady, Doulas of Color Collective, Atlanta Baby Exchange, Favor Academy of Excellence, Center for Black Women’s Wellness (CBWW), Maitu Foods, Satcher Institute at Morehouse School of Medicine, MotherToBaby Georgia (Center for Maternal Substance Abuse and Child Development),
Spellman’s GLOBE Med program, Black Mamas Matter Alliance, and OBGYN & Midwife Associates.
Know Better Live Better CEH Social Impact Campaign
The COTC held a launch event for the Know Better Live Better social impact campaign. This event included a panel discussion at the Atlanta Black Women’s Expo around environmental exposures and ways to limit exposures. Presenters included COTC staff, a Stakeholder Advisory Board member, Atlanta based physicians, and social media influencers. The social impact campaign content can be viewed on Twitter, Instagram, and Facebook by following the hashtag #KBLBATL. This campaign has been adopted by the CBWW for their Black Women’s Environmental Wellness project.
Break the Cycle of Children’s Environmental Health Disparities (BTC) Annual Conference
The COTC leveraged their partnerships and resources to enhance the annual SE PEHSU BTC conference. This included organizing a class of high school students and educators from the South Cobb High School Academy of Research and Medical Sciences magnet program to attend the conference. We were able to provide these students, the majority of which are African American, exposure to children environmental health research through a connection made by SAB member. Students also toured Emory laboratories after the conference. This class subsequently adopted the cycle of children’s environmental health research framework to develop yearlong research projects. The COTC also facilitated opportunities for community engagement via presentations by our community grantee program and networking events.
C-CHEM2 Community Grant Program
To build capacity among community organizations to conduct environmental health research and outreach we have funded four community organizations with pilot awards. These awards addressed a myriad of environmental concerns including lead exposures, exposures during breastfeeding, and exposures in early learning centers. One of our awardees, the CBWW, went on to secure additional foundation funding from the Cedar Tree Foundation.
Black Women’s Environmental Wellness Project Cedar Tree Foundation Award
In partnership with the Emory PEHSU, the Center supported the Center for Black Women’s Wellness in their development of a successful proposal to the Cedar Tree Foundation call for applications. Data from a C-CHEM2 community pilot award on environmental exposures and breastfeeding was utilized in the proposal. The project was designed to expand the work conducted in the pilot to layer environmental health concepts into the existing clinical practice and support programs offered by the CBWW for African American women. Additionally, a large component of the work involves outreach to the local community via culturally relevant social media messages which are being amplified by a network of community organizations.
Clifton School Environmental Health Early Learning Center Project
In collaboration with the HERCULES Center the COTC has engaged a local early learning center, the Clifton School, to reduce environmental exposures to children and staff in their two campuses. COTC staff conducted an initial survey of potential environmental exposures in the school and provided feedback on ways to reduce exposures. COTC staff also presented to school staff at their annual staff appreciation event. Factsheets were shared and staff were given opportunities to ask questions about ways to reduce exposures in the classroom.
Conclusions:
Project 1:
Project 1 faculty, staff, and trainees presented their work at a number of local, national, and international conferences. In total they presented 14 podium presentations, two webinars, and four poster presentations. Also, Co-Project Lead Dunlop has participated in two Stakeholder Advisory Board meetings during which updates on the progress-to-date of the Emory Children’s Study Center were shared with the community stakeholders and input was collected from the community stakeholders regarding possible ways for sharing research findings with African American communities, with a focus on women of reproductive age, as well as with the clinical providers who care for these communities.
Project 2:
Additionally, Project 2 MEND faculty, staff, and trainees presented their work at a number of local, national, and international conferences. Also, Co-Project Lead Brennan participated in Stakeholder Advisory Board meetings during which updates on the progress-to- date of the Emory Children’s Study Center were shared with the community stakeholders.
During these meetings, input was collected from the community stakeholders regarding possible ways for sharing research findings with African American communities, with a focus on women of reproductive age, as well as with the clinical providers who care for these communities.
Project 3:
Feasibility has been established to quantify more than 100 environmental chemicals detected in the metabolomics analyses. This methods development was prompted by the findings in this project which was not emphasized in publications which focused on metabolites with confirmed identities and pathways identified by pathway enrichment analyses. In addition to identified metabolites, many unidentified chemical signals from mass spectrometry analyses are associated with demographic and outcome measures. The current project did not provide resources to pursue this important question and consequently this is being pursued by the research team with other funding sources. Despite this, the developments are relevant to the current project because the analytic structure will allow future investigators to use data mining methods to explore newly identified environmental chemicals in the existing data from the population studied. Dr Jones is currently preparing a publication on these methods so that future research with other funding mechanisms can support a more global understanding of environmental exposures associated with preterm birth, pre-eclampsia, chronic stress and other measures. Other publications by the research team on environmental exposures, pregnancy and health outcomes were not directly included in this progress report because they were directly funded by other mechanisms; none-the-less, the resources available from this project have supported substantial improvement in understanding metabolomics of pregnancy and specific metabolic pathways associated with adverse outcomes.
Journal Articles: 46 Displayed | Download in RIS Format
Other center views: | All 76 publications | 30 publications in selected types | All 28 journal articles |
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Barr D. Overview and Preliminary findings of C‐CHEM2 Grand Rounds Bloomberg School of Public Health Johns Hopkins University. Baltimore, MD. April 2017. |
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Corwin E. Biological Consequences of Chronic Stress: Risk and Protective Factors across the Lifespan. The Atlanta Clinical and Translational Science Institute’s Community Engagement Research Program (DERP) Forum 2017. |
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Dunlop A. Emory Children’s Environmental Health Center: On‐going Investigation of How Environmental Exposures May Impact Preterm Birth Risk and Other Adverse Pregnancy and Child Health Outcomes. Emory Midtown Community Obstetrical Care Provider Group. Emory Midtown Office Tower, Private Practice Obstetricians 2016. |
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Fields N, Mutic A, Woldeyohannes H, McCauley L. Community Member Perspective: How to Build Authentic Relationships, Understand Value and Expertise, and Align Goals in Community‐Academic Partnerships 2017 |
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Liang D, Taibl K, Dunlop A, Barr D, Ryan P, Everson T, Juels A, Tan Y, Panuwet P, Kannan K, Marsit C, JOnes D, Eick S. Metabolic Perturbations Associated with an Exposure Mixture of Per-and Polyfluoroalkyl Substances in the Atlanta African American Maternal-Child Cohort. ENVIRONMENTAL SCIENCE & TECHNOLOGY 2023;57(43):16206-15218. |
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McCauley, L., Mutic, A., Woldeyohannes, H. “Maternal‐Child Health, the Environment, and the Microbiome”. Children’s Environmental Health Translational Research Conference:New Challenges. Arlington, VA. 7 Apr 2017. |
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Rodriguez, J. “Weight Gain and Blood Pressure in Toddlers Born Very Preterm.” Council for the Advancement of Nursing Science (CANS) Annual Meeting. Washington, DC. Sept 2016. |
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Ryan, P. Barry. “Overview of the Center for Children’s Health, the Environment, the Microbiome, and Metabolomics” The Environmental Health Science FEST. Durham, NC. 8 Dec 2016 Corwin, E., “Understanding and Utilizing Metabolomics in Research”. The University of North Carolina Chapel Hill, College of Nursing. Chapel Hill NC. 24 Mar 2017. |
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Smimov A, Jia W, Walker D, Jones D, Du X. ADAP-GC 3.2: Graphical Software Tool for Efficient Spectral Deconvolution of Gas Chromatography-High-Resolution Mass Spectrometry Metabolomics Data. JOURNAL OF PROTEOME RESEARCH 2018;17(1):470-478 |
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Carlson NS, Frediani JK, Corwin EJ, Dunlop A, Jones D. Metabolic Pathways Associated With Term Labor Induction Course in African American Women. Biological research for nursing 2020;22(2):157-68. |
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Corwin, E., Dunlop, A., Li, S., Jones, D. “Chronic stress, social disadvantage and the risk of preterm birth in African American Women” NIH Metabolomics Common Fund Supplement Workbench. Bethesda, MD. 27‐29 Sept 2016. |
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McCauley, L., United States. Cong. House. Congressional Briefing. “What the Science Says:How Environmental Protection Agency (EPA) Matters to Children's Health” Washington, DC. 5 Apr 2017. |
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McCauley, L., “Overview and Preliminary Data:Center for Children’s Health, the Environment, the Microbiome, and Metabolomics” US Environmental Protection Agency Children’s Health Protection Advisory Committee. Washington, DC. 15 Nov 2016. |
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Raskind IG, Kramer MR, Landon R. An activity space approach to assessing food‐related spatial behavior of urban African‐American women in Atlanta, GA, USA. Submitted to International Conference on Urban Health. Coimbra, Portugal. 2017. |
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Ryan, P. Barry, Barr, D., Dunlop, A., McCauley, L. “Center for Children’s Health, the Environment, Microbiome, and Metabolomics (C‐CHEM2):Project 1‐ Characterizing Exposures in an Urban Environment (CHERUB) Study Preliminary Results” Submitted to Conference of the International Society for Environmental Epidemiology. Sydney, Australia 2017. |
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Kramer MR, Raskind IG, Landon R. The ‘local trap’ or spatial entrapment? Spatial behavior among pregnant, African American women in an urban environment. Submitted to GeoMed, Porto Portugal. 2017. |
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Pittard WS, Villaveces C. Li, S. (2020). A Bioinformatics Primer to Data Science, with Examples for Metabolomics. In: Li, S. (eds) Computational Methods and Data Analysis for Metabolomics. Methods in Molecular Biology, vol 2104. Humana, New York, NY. |
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Pittard WS, Li S. (2020). The Essential Toolbox of Data Science: Python, R, Git, and Docker. In: Li, S. (eds) Computational Methods and Data Analysis for Metabolomics. Methods in Molecular Biology, vol 2104. Humana, New York, NY. |
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Chang C, Barr D, Zhang Q, Dunlop A, Smarr M, Kannan K, Panuwet P, Tangpricha V, Shi L, Liang D, Corwin E, Ryan P. Associations of single and multiple per-and polyfluoroalkyl substance PFAS exposure with vitamin D biomarkers in African American women during pregnancy. ENVIRONMENTAL RESEARCH 2021;202(111713). |
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Chang C, Ryan P, Smarr M, Kannan K, Panuwet P, Dunlop A, Corwin E, Barr D. Serum per-and polyfluoroalkyl substance PFAS concentrations and predictors of exposure among pregnant African American women in the Atlanta area, Georgia. ENVIRONMENTAL RESEARCH 2021;198(110445). |
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Corwin EJ, Hogue CJ, Pearce B, Hill CC, Read TD, Mulle J, Dunlop AL. Protocol for the Emory University African American Vaginal, Oral, and Gut Microbiome in Pregnancy Cohort Study. BMC Pregnancy and Childbirth 2017;17(1):161 (8 pp.). |
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Eatman J, Dunlop A, Barr D, Corwin E, Hill C, Brennan P, Ryan P, Panuwet P, Taibl K, Tan Y, Liang D, Eik S. Exposure to phthalate metabolites, bisphenol A, and psychosocial stress mixtures and pregnancy outcomes in the Atlanta African American maternal-child cohort. ENVIRONMENTAL RESEARCH 2023;233(116464) |
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Edwards SM, Cunningham SA, Dunlop AL, Corwin EJ. The maternal gut microbiome during pregnancy. MCN: The American Journal of Maternal/Child Nursing 2017;42(6):310-317. |
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Eick S, Tan Y, Taibl K, Ryan P, Barr D, Huls A, Eatman J, Panuwet P, D'Souza P, Yakimavets V, Lee G, Brennan P, Corwin E, Dunlop A, Liang D. Prenatal exposure to persistent and non-persistent chemical mixtures and associations with adverse birth outcomes in the Atlanta African American Maternal-Child Cohort. JOURNAL OF EXPOSURE SCIENCE AND ENVIRONMENTAL EPIDEMMIOLOGY 2023;Early Access |
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Eick S, Barr D, Brennan P, Taibl K, Tan Y, Robinson M, Kannan K, Panuwet P, Yakimavets V, Ryan P, Liang D, Dunlop A. Per-and polyfluoroalkyl substances and psychosocial stressors have a joint effect on adverse pregnancy outcomes in the Atlanta African American Maternal-Child cohort. SCIENCE OF THE TOTAL ENVIRONMENT 2023;857(2):159450. |
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Frediani JK, Naioti EA, Vos MB, Figueroa J, Marsit CJ, Welsh JA. Arsenic exposure and risk of nonalcoholic fatty liver disease (NAFLD) among U.S. adolescents and adults: an association modified by race/ethnicity, NHANES 2005-2014. Environmental Health 2018;17(1):6 (8 pp.). |
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Gardinassi LG, Xia J, Safo SE, Li S. Bioinformatics tools for the interpretation of metabolomics data. Current Pharmacology Reports 2017;3(6):374-383. |
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Gardinassi LG, Cordy RJ, Lacerda MVG, Salinas JL, Monteiro WM, Melo GC, Siqueira AM, Val FF, Tran V, Jones DP, Galinski MR, Li S. Metabolome-wide association study of peripheral parasitemia in Plasmodium vivax malaria. International Journal of Medical Microbiology 2017;307(8):533-541. |
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Kartavenka K, Panuwet P, Yakimavets V, Jaikang C, Thipubon K, D'Souza P, Barr D, Ryan P. LC-MS Quantification of Malondialdehyde-Dansylhydrazine Derivatives in Urine and Serum Samples. JOURNAL OF ANALYTICAL TOXICOLOGY 2020;44(5):470-481. |
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Li S, Dunlop AL, Jones DP, Corwin EJ. High-resolution metabolomics: review of the field and implications for nursing science and the study of preterm birth. Biological Research for Nursing 2016;18(1):12-22. |
R836153C001 (2016) R836153C003 (2016) R836153C003 (2017) |
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Mutic AD, Baker BJ, McCauley LA. Deleterious effects from occupational exposure to ethylene thiourea in pregnant women. Workplace Health and Safety 2017;65(12):595-602. |
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Mutic AD, Jordan S, Edwards SM, Ferranti EP, Thul TA, Yang I. The postpartum maternal and newborn microbiomes. MCN: The American Journal of Maternal/Child Nursing 2017;42(6):326-331. |
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Rodriguez J, Huntington-Moskos L, Johnson A, Williams S, Gulledge E, Feeley C, Rice M. Collecting biological measures for research with children and adolescents. Journal of Pediatric Health Care 2016;30(3):279-283. |
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Rodriguez J, Jordan S, Mutic A, Thul T. The neonatal microbiome: implications for neonatal intensive care unit nurses. MCN: The American Journal of Maternal/Child Nursing 2017;42(6):332-337. |
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Runkle J, Flocks J, Economos J, Dunlop AL. A systematic review of Mancozeb as a reproductive and developmental hazard. Environment International 2017; 99:29-42. |
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Swales DA, Winiarski DA, Smith AK, Stowe ZN, Newport DJ, Brennan PA. Maternal depression and cortisol in pregnancy predict offspring emotional reactivity in the preschool period. Developmental Psychobiology 2018;60(5):557-566. |
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Talibl K, Dunlop A, Barr D, Ryan P, Panuwet P, Corwin E, Eatman J, Tan Y, Liang D, Eick S. Phthalate exposure increases interferon-γ during pregnancy: The Atlanta African American Maternal-Child Cohort. SCIENCE OF THE TOTAL ENVIRONMENT 2024;916(170344) |
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Tchen R, Tan Y, Barr D, Ryan P, Tran V, Li Z, Hu Y, Smith A, Jones D, Dunlop A. Use of high-resolution metabolomics to assess the biological perturbations associated with maternal exposure to Bisphenol A and Bisphenol F among pregnant African American women. ENVIRONMENTAL INTERNATIONAL 2022;169(107530). |
R836153 (Final) |
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Yakimavets V, Qiu T, Panuwet P, D'Souza P, Brennan P, Dunlop A, Ryan P, Barr D. Simultaneous quantification of urinary tobacco and marijuana metabolites using solid-supported liquid-liquid extraction coupled with liquid chromatography tandem mass spectrometry. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 2022;1208(123378). |
R836153 (Final) |
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Zhang Z, Barr D, Dunlop A, Panuwet P, Sarnat J, Lee G, Tan Y, Corwin E, Jones D, Ryan P, Liang D. Assessment of metabolic perturbations associated with exposure to phthalates among pregnant African American women. SCIENCE OF THE TOTAL ENVIRONMENT 2022;818(151689). |
R836153 (Final) |
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Dunlop AL, Knight AK, Satten GA, Cutler AJ, Wright ML, Mitchell RM, Read TD, Mulle J, Hertzberg VS, Hill CC, Smith AK. Stability of the vaginal, oral, and gut microbiota across pregnancy among African American women:the effect of socioeconomic status and antibiotic exposure. PeerJ 2019;7:e8004. |
R836153 (2019) |
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Dunlop AL, Jordan SL, Ferranti EP, Hill CC, Patel S, Hao L, Corwin EJ, Tangpricha V. Total and Free 25-Hydroxy-Vitamin D and Bacterial Vaginosis in Pregnant African American Women. Infectious diseases in obstetrics and gynecology. 2019;2019. |
R836153 (2019) |
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Jordan, S., Baker, B., Dunn, A., Edwards, S., Ferranti, E. Mutic, A., Yang, I., & Rodriguez, J. (2017). Maternal‐child microbiome:Collection, storage, and implications for research and practice. Nursing Research, 66(2), 175‐183. |
R836153 (2017) R836153C002 (2017) |
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Rodriguez, J., Huntington-Moskos, L., Johnson, A., Williams, S., Gulledge, E., Feeley, C., & Rice, M. (2016). Collecting biological measures for research with children and adolescents. Journal of Pediatric Health Care, Doi 10.1016/j.pedhc.2015.12.007. |
R836153C002 (2017) |
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Rodriguez, J., Jordan, S., Mutic, A., & Thul, T. The neonatal microbiome:Implications for the NICU nurse. MCN:The American Journal of Maternal/Child Nursing. (in press). |
R836153 (2017) |
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Mutic, A., Jordan, S., Ferranti, E., Thul, T., Edwards, S., Yang, I. (2017). The Postpartum and Newborn Microbiomes. MCN; The American Journal of Maternal/Child Nursing. (in press). |
R836153 (2017) R836153C002 (2017) |
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Relevant Websites:
C-CHEM2 Exit , Southeastern Pediatric Environmental Health Specialty Unit Exit ,
Progress and Final Reports:
Original Abstract Subprojects under this Center: (EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).
R836153C001 Characterizing Exposures and Outcomes in an Urban Birth Cohort (CHERUB)
R836153C002 Microbiome, Environment, and Neurodevelopmental Delay (MEND)
R836153C003 Metabolic, Microbiome and Toxicant-Related Interactions (MATRIX)
The perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Conclusions drawn by the principal investigators have not been reviewed by the Agency.
Project Research Results
- 2019 Progress Report
- 2018 Progress Report
- 2017 Progress Report
- 2016 Progress Report
- Original Abstract
28 journal articles for this center