Grantee Research Project Results
2010 Progress Report: Platinum-Containing Nanomaterials: Sources, Speciation, and Toxicity in the Environment
EPA Grant Number: R833892Title: Platinum-Containing Nanomaterials: Sources, Speciation, and Toxicity in the Environment
Investigators: Schauer, James J. , Toner, Brandy M. , Shafer, Martin M.
Institution: University of Wisconsin - Madison , University of Minnesota
EPA Project Officer: Aja, Hayley
Project Period: February 1, 2009 through January 31, 2013
Project Period Covered by this Report: February 1, 2010 through January 31,2011
Project Amount: $399,406
RFA: Exploratory Research: Nanotechnology Research Grants Investigating Fate, Transport, Transformation, and Exposure of Engineered Nanomaterials: A Joint Research Solicitation - EPA, NSF, & DOE (2007) RFA Text | Recipients Lists
Research Category: Nanotechnology , Safer Chemicals
Objective:
Progress Summary:
i. Total metal (Pt, Pd, Rh and 45 other elements) content in the particulates is determined by magnetic-sector ICP-MS after complete dissolution of the particulates (microwave assisted acid digestion in Teflon bombs).
ii. A battery of extraction fluids are used to assess the soluble/mobile fraction of Pt (and other elements) in the particulates: (a) high purity water (MQ) is used as a reference fluid, (b) two physiologically relevant fluids - Gambles and a surrogate macrophage vacuole solution, simulate availability to biological systems, (c) 1 N HCl is the preferred matrix for extraction/preservation of chloroplatinate species, (d) methanol is applied to selected samples to access binding sites sequestered in the hydrophobic soot matrix. Total extractable Pt (and other metals) in each fluid is determined by magnetic-sector ICP-MS, after filtration at 0.22 micron.
iii. Colloidal/nano and dissolved species are physically separated by ultrafiltration at 10 kDa in all extraction fluids, and then each fraction is subjected to SF-ICP-MS.
iv. Anionic species (e.g., chloroplatinates) are physically separated from neutral and cationic species using DEAE Ion Chromatography, and each fraction is then characterized by SF-ICP-MS.
v. Soluble ions (nitrate, chloride, sulfate, ammonium) and soluble organic carbon are also measured.
Future Activities:
References:
- Nachtigall, D.; Artelt, S.; Wunsch, G. J. Chromatogr. A. 775 (1997) 197-210.
- Nischwitz, V.; Michalke, B.; Kettrup, A. J. Chromatogr. A. 1016 (2003) 223.
- Nischwitz, V.; Michalke, B.; Kettrup,A. Anal. Chim. Acta. 521 (2004) 87-93.
Journal Articles:
No journal articles submitted with this report: View all 7 publications for this projectSupplemental Keywords:
Progress and Final Reports:
Original AbstractThe perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Conclusions drawn by the principal investigators have not been reviewed by the Agency.