Grantee Research Project Results

2006 Progress Report: Environmentally Superior Soybean Genome Development

EPA Grant Number: R829479C027
Subproject: this is subproject number 027 , established and managed by the Center Director under grant R829479
(EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).
Center: The Consortium for Plant Biotechnology Research, Inc., Environmental Research and Technology Transfer Program
Center Director: Schumacher, Dorin
Title: Environmentally Superior Soybean Genome Development
Investigators: Pantalone, Vincent R
Institution: University of Tennessee
EPA Project Officer: Aja, Hayley
Project Period: October 1, 2004 through September 30, 2007 (Extended to December 31, 2007)
Project Period Covered by this Report: October 1, 2005 through September 30, 2006
RFA: The Consortium for Plant Biotechnology Research, Inc., Environmental Research and Technology Transfer Program (2001) RFA Text |  Recipients Lists
Research Category: Hazardous Waste/Remediation , Targeted Research

Objective:

The long-term objective is to develop a commercially acceptable, superior quality, high yielding soybean variety with low seed phytate. The specific aim of this proposed research is to utilize SSR molecular genetic markers distributed among all 20 soybean linkage groups to facilitate genome recovery, and to use low-phytate markers Satt237 and Satt561 for dual marker assisted selection for gene transfer of the low phytate trait to a superior quality, high yielding conventional soybean variety, 5601T.

Progress Summary:

We made 1,452 individual cross-hybridizations utilizing pollen from second generation backcross (BC2F1) individuals confirmed from our DNA analyses as true double-heterozygotes. Each selected donor contained one copy of the low-phytate type allele at both the molecular linkage group (MLG) N Satt237 locus and at the MLG L Satt561 locus, thereby enabling dual marker-assisted selection for gene transfer of the low phytate trait to proceed.

This intensive effort resulted in the collection of 547 putative BC3F1 seeds, selections of which were grown in Knoxville, Tennessee, during 2006. DNA analyses revealed that a fourth backcross generation would be needed to obtain strong recurrent parent genome recovery. We selected the four best individuals to serve as new donors, which had the greatest DNA homology with the 5601T recurrent parent genome and which were also confirmed as true double-heterozygotes for the two low-phytate loci. Those plants are currently approaching physiological maturity and the resulting BC4F1 hybrid seed will be collected for generation advancement.

Concurrently, we pursued our plan to derive selected individuals homozygous for both of the recessive low-phytate loci as offspring from the BC1F1 and from the BC2F1 populations. We grew 40 rows of BC1F2 plants and 10 rows of BC2F2 plants, each 4.5 m in length, in Homestead, Florida, during the winter months of early 2006 and grew their progeny in Knoxville, Tennessee, during the summer through autumn 2006 season. Selections will be made for the expression of low-phytate based on chemical analyses of seeds from among hundreds of these individuals.

Future Activities:

We plan to expand our screening of available polymorphic markers to better characterize the capture of recurrent parent genome. Seeds from selected plants will be measured for inorganic phosphorous concentration to validate that not just linked molecular marker loci, but also low-phytate trait expression inherited at the BC1, BC2, and BC3 stages for further trait transmission to the BC3 stage.

Near-infrared spectroscopy will be performed on seeds to determine protein and oil concentration and amino acid composition of the protein. Methyl esters of the oil will be prepared for gas chromatographic analyses for oil fatty acid composition determination. These seed quality analyses will ensure that the quality of the final end product will be well characterized.

DNA will be isolated from BC4F1 plants and analyzed with molecular markers to determine recurrent parent genome recovery and double-heterozygous existence of low-phytate loci.

Seeds from the best BC4F1 plants will be grown, and selected progeny that express the pure state double-recessive Satt237 and Satt561 homozygous low-phytate loci will be increased for future pure-line development, targeting testing for commercialization of a new low-phytate soybean variety.

In addition, progeny from BC1, BC2, and BC3 generations that test positive for the low-phytate trait will be carried forward as genetic resources for further low-phytate population development.

Marker-assisted selection for the low-phytate trait will be confirmed in another genetic background to validate the concept and to simultaneously improve soybean protein meal and plant disease resistance.

Journal Articles:

No journal articles submitted with this report: View all 7 publications for this subproject

Supplemental Keywords:

phosphorous, phytate, poultry nutrition, swine nutrition, confined animal feeding operations (CAFOs),, RFA, Scientific Discipline, TREATMENT/CONTROL, Sustainable Industry/Business, Genetics, Sustainable Environment, Treatment Technologies, Technology, Technology for Sustainable Environment, New/Innovative technologies, Agricultural Engineering, bioengineering, transgenic plants, plant genes, biotechnology, plant biotechnology, cloning, bioremediation

Progress and Final Reports:

Original Abstract

2005

2007

Final

Main Center Abstract and Reports:

R829479    The Consortium for Plant Biotechnology Research, Inc., Environmental Research and Technology Transfer Program

Subprojects under this Center: (EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).
R829479C001 Plant Genes and Agrobacterium T-DNA Integration
R829479C002 Designing Promoters for Precision Targeting of Gene Expression
R829479C003 aka R829479C011 Biological Effects of Epoxy Fatty Acids
R829479C004 Negative Sense Viral Vectors for Improved Expression of Foreign Genes in Insects and Plants
R829479C005 Development of Novel Plastics From Agricultural Oils
R829479C006 Conversion of Paper Sludge to Ethanol
R829479C007 Enhanced Production of Biodegradable Plastics in Plants
R829479C008 Engineering Design of Stable Immobilized Enzymes for the Hydrolysis and Transesterification of Triglycerides
R829479C009 Discovery and Evaluation of SNP Variation in Resistance-Gene Analogs and Other Candidate Genes in Cotton
R829479C010 Woody Biomass Crops for Bioremediating Hydrocarbons and Metals
R829479C011 Biological Effects of Epoxy Fatty Acids
R829479C012 High Strength Degradable Plastics From Starch and Poly(lactic acid)
R829479C013 Development of Herbicide-Tolerant Energy and Biomass Crops
R829479C014 Identification of Receptors of Bacillus Thuringiensis Toxins in Midguts of the European Corn Borer
R829479C015 Coordinated Expression of Multiple Anti-Pest Proteins
R829479C016 A Novel Fermentation Process for Butyric Acid and Butanol Production from Plant Biomass
R829479C017 Molecular Improvement of an Environmentally Friendly Turfgrass
R829479C018 Woody Biomass Crops for Bioremediating Hydrocarbons and Metals. II.
R829479C019 Transgenic Plants for Bioremediation of Atrazine and Related Herbicides
R829479C020 Root Exudate Biostimulation for Polyaromatic Hydrocarbon Phytoremediation
R829479C021 Phytoremediation of Heavy Metal Contamination by Metallohistins, a New Class of Plant Metal-Binding Proteins
R829479C022 Development of Herbicide-Tolerant Energy and Biomass Crops
R829479C023 A Novel Fermentation Process for Butyric Acid and Butanol Production from Plant Biomass
R829479C024 Development of Vectors for the Stoichiometric Accumulation of Multiple Proteins in Transgenic Crops
R829479C025 Chemical Induction of Disease Resistance in Trees
R829479C026 Development of Herbicide-Tolerant Hardwoods
R829479C027 Environmentally Superior Soybean Genome Development
R829479C028 Development of Efficient Methods for the Genetic Transformation of Willow and Cottonwood for Increased Remediation of Pollutants
R829479C029 Development of Tightly Regulated Ecdysone Receptor-Based Gene Switches for Use in Agriculture
R829479C030 Engineered Plant Virus Proteins for Biotechnology