Grantee Research Project Results
Final Report: Genetics of Phthalate and Bisphenol A Risk in Minority Populations (Individual Susceptibility)
EPA Grant Number: R831711C003Subproject: this is subproject number 003 , established and managed by the Center Director under grant R831711
(EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).
Center: Center for Research on Early Childhood Exposure and Development in Puerto Rico
Center Director: Alshawabkeh, Akram
Title: Genetics of Phthalate and Bisphenol A Risk in Minority Populations (Individual Susceptibility)
Investigators: Wetmur, James G. , Wolff, Mary S. , Chen, Jia
Institution: Mount Sinai School of Medicine
EPA Project Officer: Callan, Richard
Project Period: November 1, 2003 through October 31, 2008 (Extended to October 31, 2010)
RFA: Centers for Children's Environmental Health and Disease Prevention Research (2003) RFA Text | Recipients Lists
Research Category: Children's Health , Human Health
Objective:
- Determine HDL levels for the existing birth cohort and carry out a detailed genotype/phenotype reanalysis (a continuation of PON1 phenotype/genotype studies from the previous funding period). Genotype and haplotype new members of the extended birth cohort for PON1 polymorphisms. Collaborate with Project 2 to examine PON1 genotype and phenotype effects on developmental outcomes.
-
Genotype a common missense polymorphism in human UGT2B7 in our population of African-Americans, Caucasians and Hispanics and its association with urinary metabolites. Identify and assess common promoter, coding region and splice-site polymorphisms in human UGT2B7 in our African-Americans, Caucasians and Hispanics, establish the common haplotypes, and their association with exposure. Collaborate with Project 2 to examine human UGT2B7 genotype/haplotype effects on developmental outcomes.
- Examine salivary lipase activity as a biomarker for conversion of phthalate diesters to phthalate monoesters. Determine expression levels of the three linked human lipase genes in human salivary glands. Determine racial/ethnic differences in the frequencies of the common polymorphisms in the salivary human lipase gene(s) and in salivary lipase activity. Examine salivary lipase activity in relation to urinary levels of phthalate metabolites. Examine salivary lipase polymorphisms and haplotypes in relation to urinary levels of phthalate metabolites. Obtain pilot data on the extent of glucuronidation of phthalate monoesters in urine. Collaborate with the epidemiology project (Project 2-R831711C002) to examine association of lipase genotype and phenotype with child developmental outcomes.
Summary/Accomplishments (Outputs/Outcomes):
Associations between lipase genotypes and biomarkers were evaluated by a two-level model where the heterozygote and rare homozygote contributions were combined. All analyses were adjusted for race/ethnicity and creatinine. We observed strong association with HMWP and LMWP that differed by genotype. Although the SNP associations for the lipase genes were found after adjustment for race/ethnicity, to reduce the possibility of confounding by population stratification, we examined the haplotype structure and associations for the two lipase genes. Association analysis by haplotype adjusted for creatinine (restricted to >20 mg/dl) revealed strong associations.
Conclusions:
All publications for this project are reported under the Final Report for the Center (see Final Report for Grant No. R831711)
Journal Articles:
No journal articles submitted with this report: View all 93 publications for this subprojectSupplemental Keywords:
Biochemistry, Chemicals, childhood development, children's environmental health, Children's Health, children's vulnerablity, endocrine disrupting chemicals, endocrine disruptors, Environmental Chemistry, Environmental Exposure & Risk, environmental health, environmental management, exposure pathways, exposure studies, fast food, Health, Health Risk Assessment, human health, lipase, neurodevelopment, neurodevelopmental toxicity, obesity, paraoxonase, pesticide exposure, pesticides, phthalates, pollutants/toxics, RFA, Risk Assessment, Scientific Discipline, RFA, Health, Scientific Discipline, ENVIRONMENTAL MANAGEMENT, POLLUTANTS/TOXICS, Environmental Chemistry, Health Risk Assessment, Chemicals, Endocrine Disruptors - Environmental Exposure & Risk, endocrine disruptors, Biochemistry, Children's Health, Endocrine Disruptors - Human Health, Risk Assessment, environmental health, pesticide exposure, childhood development, pesticides, phtalates, endocrine disrupting chemicals, exposure studies, Human Health Risk Assessment, children's vulnerablity, neurodevelopmental toxicity, genetic polymorphisms, children's environmental health, exposure pathwaysRelevant Websites:
http://www.mountsinai.org/patient-care/service-areas/children/areas-of-care/childrens-environmental-health-center/ Exit
Progress and Final Reports:
Original AbstractMain Center Abstract and Reports:
R831711 Center for Research on Early Childhood Exposure and Development in Puerto Rico Subprojects under this Center: (EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).
R831711C001 Growing Up Healthy in East Harlem (Community-Based Participatory Research)
R831711C002 Pesticides, Endocrine Disruptors, Childhood Growth and Development (Birth Cohort)
R831711C003 Genetics of Phthalate and Bisphenol A Risk in Minority Populations (Individual Susceptibility)
The perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Conclusions drawn by the principal investigators have not been reviewed by the Agency.
Project Research Results
- 2009
- 2008
- 2007 Progress Report
- 2006 Progress Report
- 2005 Progress Report
- 2004 Progress Report
- Original Abstract
52 journal articles for this subproject
Main Center: R831711
256 publications for this center
120 journal articles for this center