Grantee Research Project Results
Characterization of Beggiatoa in Black Band Disease of Scleractinian Corals
EPA Grant Number: U915620Title: Characterization of Beggiatoa in Black Band Disease of Scleractinian Corals
Investigators: Viehman, Tiffany S.
Institution: Florida International University
EPA Project Officer: Hahn, Intaek
Project Period: August 1, 1999 through August 1, 2001
Project Amount: $56,581
RFA: STAR Graduate Fellowships (1999) RFA Text | Recipients Lists
Research Category: Fellowship - Microbiology , Biology/Life Sciences , Academic Fellowships
Objective:
The overall objective of this research project is to characterize the ecological physiology of the sulfide-oxidizing bacterium, Beggiatoa, in black band disease of scleractinian corals. The specific objective of this research project is to address the contribution of Beggiatoa to the sulfur and carbon cycles that are generated and maintained by the microbial members of the black band disease consortium.
Approach:
Samples of black band disease are collected from infected coral colonies in the Florida Keys and Caribbean. Black band Beggiatoa spp. are in culture at the laboratory, and pure culture isolations are in progress. Metabolic capabilities—including sulfur and carbon source utilization—of black band Beggiatoa will be studied using microelectrodes sensitive to pH, oxygen, and sulfide. This approach allows the investigation of changes in oxygen and sulfide flux both in vitro (in laboratory gradient cultures) and in situ (underwater) in active black band disease on the coral. The location of Beggiatoa in vertical, sulfide-oxygen gradients of black band disease will be examined with regard to the other members of the microbial consortium. Investigations of Beggiatoa motilities also will be addressed using light manipulations. The 16s rRNA sequences from black band disease Beggiatoa samples will be compared to those of known cultures of Beggiatoa found in both marine and freshwater sulfide-interface environments.
Supplemental Keywords:
fellowship, Beggiatoa, black band disease, coral disease, coral reef, Florida Keys, Caribbean., RFA, Ecosystem Protection/Environmental Exposure & Risk, Scientific Discipline, Water, Aquatic Ecosystem, Restoration, Aquatic Ecosystems & Estuarine Research, Aquatic Ecosystem Restoration, Oceanography, Biochemistry, Ecology and Ecosystems, metabolism, marine biology, marine biogeochemistry, estrogen, bioaccumulation, aquatic ecosystems, Scleractinian corals, Black Band Disease, sulfide oxidizing bacteriumProgress and Final Reports:
The perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Conclusions drawn by the principal investigators have not been reviewed by the Agency.