Grantee Research Project Results

Vascular Enzyme (Semicarbazide-Sensitive Amine Oxidase) -Mediated Methylamine Toxicity

EPA Grant Number: U915014
Title: Vascular Enzyme (Semicarbazide-Sensitive Amine Oxidase) -Mediated Methylamine Toxicity
Investigators: Langford, Shannon D.
Institution: The University of Texas Medical Branch - Galveston
EPA Project Officer: Packard, Benjamin H
Project Period: January 1, 1996 through December 1, 1996
Project Amount: $102,000
RFA: STAR Graduate Fellowships (1996) RFA Text |  Recipients Lists
Research Category: Fellowship - Toxicology , Academic Fellowships , Human Health

Objective:

The objective of this research project is to determine the nature of enzyme-mediated vascular injury resulting from exposure to the environmental toxin methylamine.

Approach:

Cultured rat vascular smooth muscle cells were utilized as the experimental platform for determination of cytotoxicity of methylamine. MA. Cytotoxicity was measured via vital dye inclusion assays, MTT mitochondrial function assay, and clonigenic assay according to the methods described by Borenfreund, et al., 1988; Mosmann, 1983; and Freshney, 1987. Semicarbazide-sensitive amine oxidase (SSAO) activity was determined by employing a previously described radiometric ¹⁴C-labeled benzylamine substrate. (Lewinsohn, et al., 1978).

Supplemental Keywords:

fellowship, toxicity, enzyme mediated, cytotoxicity, vascular injury, methylamine, enzyme-mediated toxicity., Scientific Discipline, Health, ENVIRONMENTAL MANAGEMENT, Health Risk Assessment, Risk Assessments, Biochemistry, Risk Assessment, animal model, environmental risks, methylamine, environmental mutagens, toxicity, vascular dysfunction, animal bioassays, cytotoxicity, exposure assessment

Progress and Final Reports:

Final