Grantee Research Project Results
Final Report: A Continuation: Humate-Induced Remediation of Petroleum Contaminated Surface Soils
EPA Grant Number: R827015C022Subproject: this is subproject number 022 , established and managed by the Center Director under grant R827015
(EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).
Center: HSRC (1989) - Northeast HSRC
Center Director: Sidhu, Sukh S.
Title: A Continuation: Humate-Induced Remediation of Petroleum Contaminated Surface Soils
Investigators: Nanny, Mark A. , Andrusevich, Vladimir E. , Philp, R. Paul , Ulrich, Glenn
Institution: University of Oklahoma
EPA Project Officer: Aja, Hayley
Project Period: September 4, 2002 through September 4, 2003
RFA: Integrated Petroleum Environmental Consortium (IPEC) (1999) RFA Text | Recipients Lists
Research Category: Hazardous Waste/Remediation , Targeted Research
Objective:
The objectives of this proposed research were to: (1) measure the biodegradation and bioavailability of 14C-labeled heptadecane that is adsorbed strongly to humate; (2) examine the adsorption of gasoline, gasoline oxygenates as a part of gasoline itself, and diesel fuel to humates, as well as their biodegradation in soil microcosms; and (3) assess humate-induced remediation on a pilot-scale by conducting ex situ tests of soils contaminated with crude oil, diesel fuel, and gasoline.
Summary/Accomplishments (Outputs/Outcomes):
Several trips with extensive sample collecting were performed at the Sunoco refinery at Tulsa, Oklahoma. More than 20 samples of contaminated soils were collected there with the assistance of Sunoco personnel, followed by analyses of dichloromethane-acetone extracts with gas chromatography (GC) and gas chromatography- mass spectrometry (GC/MS) methods. Unfortunately, no samples contaminated with relatively new spills of crude oil or refined petroleum products were found.
The adsorption of diesel fuel to Earthgree Menefee humate (Cuba, New Mexico), which was used in all our experiments during this project, was analyzed using a gravimetric method. The total amount of adsorbed diesel fuel was calculated as a difference between final loss for humate contaminated with diesel fuel and final loss for humate itself after complete dichloromethane and dichloromethane-methanol extractions. Results of this experiment demonstrated that neither freeze-dried humate (freeze-dried to remove surface moisture), nor as is humate retained hydrocarbons of diesel fuel range. This conclusion was also supported by pyrolysis-GC study of n-alkane and n-alkene concentrations in the solid residues of humate after extraction. The ratios of n-alkanes to n-alkenes did not show its increase for any of the eight samples studied.
Approximately 150 kg of each of test soil (pristine, gasoline- , diesel fuel-, and crude oil-contaminated), required to create 448 microcosms, was collected from a depth of approximately 3 to 5 feet at the Waggoner site (an abandoned oil refinery), located 30 miles west of Wichita Falls, Texas. Access to the site, as well as identification of contaminated regions (type and amount of contaminant), was provided by Surbec. Surbec also loaned us coring equipment. The results of GCMS analyses of dichloromethane-acetone extracts from all three types of contaminated soils convincingly demonstrated that they contain appropriate concentrations of moderately degraded petroleum hydrocarbons, thus meeting criteria required for our investigation. Analysis of pristine soil shows that it is practically free of petroleum hydrocarbons and therefore will serve as a control in both microcosm and field experiments. Microcosm experiments are currently in a progress. Three sets of duplicate samples (56 microcosms in each set) for time 0, day 15, and day 30 were sacrificed and extracted for analyses.
A pilot-scale ex situ experiment was implemented at the Waggoner site with the help of Waggoner Estate personnel. Totally, 20 windrows consisting of 4 types of soil (pristine, gasoline-, diesel fuel-, and crude oil-contaminated) were deployed. The windrow’s size is approximately 9.5 x 3 x 1 ft. Five windrows for each type of soil represent control windrow, a humate-added windrow, a fertilizer-added windrow, a humate plus fertilizer-added windrow, and microbial activity inhibitor-added windrow. Samples were collected at time 0 and day 30 in the amount of 120 samples each time. At each sampling time, three approximately 200 g samples from the surface and three approximately 200 g samples from the subsurface of each windrow were sacrificed. Selected samples are in process of Soxhlet extraction to isolate dichloromethane-MeOH soluble hydrocarbons. A microcosm experiment is also currently in progress. Two sets of duplicate samples (56 microcosms in each set) for 2 and 3 months were sacrificed and are in process of extractions for GC and GC/MS analyses. Selected data of GC/MS analyses of diesel fuel- and gasoline-contaminated soils demonstrate certain diversity in compositions of extracted hydrocarbons.
Journal Articles:
No journal articles submitted with this report: View all 2 publications for this subprojectSupplemental Keywords:
gasoline, diesel fuel, crude oil, microcosm, humate, adsorption,, RFA, Scientific Discipline, INTERNATIONAL COOPERATION, Waste, Water, TREATMENT/CONTROL, Waste Treatment, Contaminated Sediments, Remediation, Environmental Chemistry, Hazardous Waste, Environmental Monitoring, Ecological Risk Assessment, Hazardous, Environmental Engineering, hazardous waste management, hazardous waste treatment, sediment treatment, risk assessment, advanced treatment technologies, cleanup, remediation technologies, contaminated sediment, bioavailability, humate induced remediation, metal release, treatment, hazadous waste streams, surface soils, oil spillsRelevant Websites:
Main Center Abstract and Reports:
R827015 HSRC (1989) - Northeast HSRC Subprojects under this Center: (EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).
R827015C001 Evaluation of Road Base Material Derived from Tank Bottom Sludges
R827015C002 Passive Sampling Devices (PSDs) for Bioavailability Screening of Soils Containing Petrochemicals
R827015C003 Demonstration of a Subsurface Drainage System for the Remediation of Brine-Impacted Soil
R827015C004 Anaerobic Intrinsic Bioremediation of Whole Gasoline
R827015C005 Microflora Involved in Phytoremediation of Polyaromatic Hydrocarbons
R827015C006 Microbial Treatment of Naturally Occurring Radioactive Material (NORM)
R827015C007 Using Plants to Remediate Petroleum-Contaminated Soil
R827015C008 The Use of Nitrate for the Control of Sulfide Formation in Oklahoma Oil Fields
R827015C009 Surfactant-Enhanced Treatment of Oil-Contaminated Soils and Oil-Based Drill Cuttings
R827015C010 Novel Materials for Facile Separation of Petroleum Products from Aqueous Mixtures Via Magnetic Filtration
R827015C011 Development of Relevant Ecological Screening Criteria (RESC) for Petroleum Hydrocarbon-Contaminated Exploration and Production Sites
R827015C012 Humate-Induced Remediation of Petroleum Contaminated Surface Soils
R827015C013 New Process for Plugging Abandoned Wells
R827015C014 Enhancement of Microbial Sulfate Reduction for the Remediation of Hydrocarbon Contaminated Aquifers - A Laboratory and Field Scale Demonstration
R827015C015 Locating Oil-Water Interfaces in Process Vessels
R827015C016 Remediation of Brine Spills with Hay
R827015C017 Continuation of an Investigation into the Anaerobic Intrinsic Bioremediation of Whole Gasoline
R827015C018 Using Plants to Remediate Petroleum-Contaminated Soil
R827015C019 Biodegradation of Petroleum Hydrocarbons in Salt-Impacted Soil by Native Halophiles or Halotolerants and Strategies for Enhanced Degradation
R827015C020 Anaerobic Intrinsic Bioremediation of MTBE
R827015C021 Evaluation of Commercial, Microbial-Based Products to Treat Paraffin Deposition in Tank Bottoms and Oil Production Equipment
R827015C022 A Continuation: Humate-Induced Remediation of Petroleum Contaminated Surface Soils
R827015C023 Data for Design of Vapor Recovery Units for Crude Oil Stock Tank Emissions
R827015C024 Development of an Environmentally Friendly and Economical Process for Plugging Abandoned Wells
R827015C025 A Continuation of Remediation of Brine Spills with Hay
R827015C026 Identifying the Signature of the Natural Attenuation of MTBE in Goundwater Using Molecular Methods and "Bug Traps"
R827015C027 Identifying the Signature of Natural Attenuation in the Microbial
Ecology of Hydrocarbon Contaminated Groundwater Using Molecular Methods and
"Bug Traps"
R827015C028 Using Plants to Remediate Petroleum-Contaminated Soil: Project Continuation
R827015C030 Effective Stormwater and Sediment Control During Pipeline Construction Using a New Filter Fence Concept
R827015C031 Evaluation of Sub-micellar Synthetic Surfactants versus Biosurfactants for Enhanced LNAPL Recovery
R827015C032 Utilization of the Carbon and Hydrogen Isotopic Composition of Individual Compounds in Refined Hydrocarbon Products To Monitor Their Fate in the Environment
R830633 Integrated Petroleum Environmental Consortium (IPEC)
R830633C001 Development of an Environmentally Friendly and Economical Process for Plugging Abandoned Wells (Phase II)
R830633C002 A Continuation of Remediation of Brine Spills with Hay
R830633C003 Effective Stormwater and Sediment Control During Pipeline Construction Using a New Filter Fence Concept
R830633C004 Evaluation of Sub-micellar Synthetic Surfactants versus Biosurfactants for Enhanced LNAPL Recovery
R830633C005 Utilization of the Carbon and Hydrogen Isotopic Composition of Individual Compounds in Refined Hydrocarbon Products To Monitor Their Fate in the Environment
R830633C006 Evaluation of Commercial, Microbial-Based Products to Treat Paraffin Deposition in Tank Bottoms and Oil Production Equipment
R830633C007 Identifying the Signature of the Natural Attenuation in the Microbial Ecology of Hydrocarbon Contaminated Groundwater Using Molecular Methods and “Bug Traps”
R830633C008 Using Plants to Remediate Petroleum-Contaminated Soil: Project Continuation
R830633C009 Use of Earthworms to Accelerate the Restoration of Oil and Brine Impacted Sites
The perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Conclusions drawn by the principal investigators have not been reviewed by the Agency.
Project Research Results
Main Center: R827015
120 publications for this center
16 journal articles for this center