Grantee Research Project Results
Regulation of Embryonic Neuronal Development by Chemical Mixtures from Brick, NJ
EPA Grant Number: R829359Title: Regulation of Embryonic Neuronal Development by Chemical Mixtures from Brick, NJ
Investigators: Reinisch, Carol L. , Kreiling, Jill , Stephens, Raymond
Current Investigators: Reinisch, Carol L. , Cox, Rachel L. , Kreiling, Jill
Institution: Marine Biological Laboratory
EPA Project Officer: Hahn, Intaek
Project Period: July 1, 2001 through June 30, 2004 (Extended to September 23, 2006)
Project Amount: $749,333
RFA: Complex Chemical Mixtures (2000) RFA Text | Recipients Lists
Research Category: Hazardous Waste/Remediation , Safer Chemicals , Land and Waste Management
Description:
We will use Spisula as a rapid, reproducible, and exquisitely sensitive model to define the mechanism of action of mixtures of neurotoxic substances on the p53 family of genes during development. We will use bromoform, chloroform, and tectrachloroethylene(PCE), substances of high relevance in the study of autism in Brick, NJ.
Approach:
Define the impact of bromoform/chloroform and / PCE on the p53 family of genes during development. Use amounts of chemicals found in wells in Brick NJ, a site that is the focus of current studies by the EPA. Analyze neuropeptide synthesis and neurospecific protein expression by northern and western blot analysis.
Expected Results:
We expect that mixtures of bromoform, chloroform, and PCE will impact neuronal development and p53 expression in Spisula. By examining the p53 family, we are focusing on the genetic target of chemicals in the environment. Any perturbations in p53 in any species should serve as a critically important biomarker for chemical exposure. We have selected chemical mixtures which impair neural function. Together, these data should have relevance to state and federal agencies responsible for risk assessment and management.
Publications and Presentations:
Publications have been submitted on this project: View all 2 publications for this projectJournal Articles:
Journal Articles have been submitted on this project: View all 1 journal articles for this projectSupplemental Keywords:
biomarkers, brain function, neurotransmitters., RFA, Scientific Discipline, Geographic Area, Waste, Hazardous, Fate & Transport, State, chemical mixtures, Environmental Chemistry, Hazardous Waste, Ecology and Ecosystems, environmental transport and fate, hazardous organic substances, bioavailability, contaminated sediments, analytical models, fate and transport , biodegradation, complex mixtures, fate and transport, effects assessment, embryonic neuronal development, PCE, chemical kinetics, contaminant transport models, genotoxicity, contaminated soils, hazardous chemicalsProgress and Final Reports:
The perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Conclusions drawn by the principal investigators have not been reviewed by the Agency.