Grantee Research Project Results
2000 Progress Report: Anaerobic Intrinsic Bioremediation of Whole Gasoline
EPA Grant Number: R827015C004Subproject: this is subproject number 004 , established and managed by the Center Director under grant R827015
(EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).
Center: HSRC (1989) - Northeast HSRC
Center Director: Sidhu, Sukh S.
Title: Anaerobic Intrinsic Bioremediation of Whole Gasoline
Investigators: Suflita, Joseph
Current Investigators: Thoma, Greg , Beyrouty, Craig , Wolf, Duane
Institution: University of Oklahoma
EPA Project Officer: Aja, Hayley
Project Period: February 1, 1999 through January 31, 2000 (Extended to June 30, 2001)
Project Period Covered by this Report: February 1, 1999 through January 31, 2000
Project Amount: Refer to main center abstract for funding details.
RFA: Integrated Petroleum Environmental Consortium (IPEC) (1999) RFA Text | Recipients Lists
Research Category: Hazardous Waste/Remediation , Targeted Research
Objective:
Conduct investigation into the prospect for whole gasoline degradation under anaerobic conditions.
Progress Summary:
We have been continually monitoring the susceptibility of whole gasoline to decay under methanogenic, sulfate- reducing, and nitrate-reducing conditions in ongoing, extended incubations. Although high rates of endogenous methanogenesis and sulfate reduction have been observed in the first calendar year, to date, no stimulation has been seen due to the initial 10 l gasoline amendment. Nitrate-amended microcosms have demonstrated little nitrate reduction or hydrocarbon biodegradation. These and previous findings indicate that this terminal electron acceptor does not play a significant role in petroleum metabolism at this site.
We have begun to analyze the extensive hydrocarbon data from the first six months of these incubations. There are significant differences between the amounts of analytes detected in the aqueous phases under different terminal electron accepting-conditions; this may reflect differential partition to the solids.
Overall, our preliminary findings indicate much greater biodegradation under sulfate-reducing conditions than under methanogenic conditions by the indigenous microbiota at the Ft. Lupton site. A wide range of aromatic, alicyclic, and alkane substrates were degraded under sulfate-reducing conditions, surprisingly with most of the degradation occurring in the first 40 days of incubation. Branched alkanes were much more resistant to biodegradation than straight chain alkanes. Also, BTEX compoundswere selectively degraded under sulfate-reducing and methanogenic conditions. Of the xylene isomers, the meta- and ortho- isomers were more labile than the para-isomerunder sulfate-reducing conditions. Under methanogenic conditions, the para- and meta-isomers were preferred over the orth-isomer. Benzene clearly appears to be the most recalctrant of the BTEX isomers.
Parallel experiments, examining the fate of alkane and alicyclic compounds as sole substrates have continued. As in the gasoline experiment, high rates of endogenous methanogenesis and sulfate-reduction have largely masked any stimulation that may have loccurred due to the substrate amendments. However, we have transferred the original incubations into media, and this has resulted in both lower endogenous rates of methanogenesis and sulfate reduction as well as significant stimulation of these process in pentane-, hexane-, octane-, undecane-, and pentadecane- amended. These experiments reveal the ability of the resident microbiota to biodegraded these components commonly found in gasoline and gas condensate and confirm the results found with gasoline as a complex substrate.
Future Activities:
Based upon the residual hydrocarbons remaining in the gasoline incubations after one year of incubation, we requested and were granted a no-cost extension in order to continue monitoring gasoline biodegradation in these incubations over an additional year.
Journal Articles:
No journal articles submitted with this report: View all 11 publications for this subprojectSupplemental Keywords:
hydrocarbon, biodegradation, bioremediation, methanogenic, sulfate-reducing, nitrate-reducing, BTEX., RFA, Scientific Discipline, Geographic Area, Waste, Ecosystem Protection/Environmental Exposure & Risk, Bioavailability, Remediation, State, Chemistry, Northwest, Civil/Environmental Engineering, Environmental Microbiology, Microbiology, Biochemistry, Hazardous Waste, Oil Spills, Bioremediation, Biology, Engineering, Environmental Engineering, Groundwater remediation, Hazardous, risk assessment, gasoline, anaerobic treatment, biodegradation, electron acceptor, anaerobic biodegradation, anaerobic bioconversion, hydrocarbons, risk assessments, Ft. Lupton, CO, biological markers, anaerobic bioremediation, anaerobic biotransformation, Colorado (CO)Progress and Final Reports:
Original AbstractMain Center Abstract and Reports:
R827015 HSRC (1989) - Northeast HSRC Subprojects under this Center: (EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).
R827015C001 Evaluation of Road Base Material Derived from Tank Bottom Sludges
R827015C002 Passive Sampling Devices (PSDs) for Bioavailability Screening of Soils Containing Petrochemicals
R827015C003 Demonstration of a Subsurface Drainage System for the Remediation of Brine-Impacted Soil
R827015C004 Anaerobic Intrinsic Bioremediation of Whole Gasoline
R827015C005 Microflora Involved in Phytoremediation of Polyaromatic Hydrocarbons
R827015C006 Microbial Treatment of Naturally Occurring Radioactive Material (NORM)
R827015C007 Using Plants to Remediate Petroleum-Contaminated Soil
R827015C008 The Use of Nitrate for the Control of Sulfide Formation in Oklahoma Oil Fields
R827015C009 Surfactant-Enhanced Treatment of Oil-Contaminated Soils and Oil-Based Drill Cuttings
R827015C010 Novel Materials for Facile Separation of Petroleum Products from Aqueous Mixtures Via Magnetic Filtration
R827015C011 Development of Relevant Ecological Screening Criteria (RESC) for Petroleum Hydrocarbon-Contaminated Exploration and Production Sites
R827015C012 Humate-Induced Remediation of Petroleum Contaminated Surface Soils
R827015C013 New Process for Plugging Abandoned Wells
R827015C014 Enhancement of Microbial Sulfate Reduction for the Remediation of Hydrocarbon Contaminated Aquifers - A Laboratory and Field Scale Demonstration
R827015C015 Locating Oil-Water Interfaces in Process Vessels
R827015C016 Remediation of Brine Spills with Hay
R827015C017 Continuation of an Investigation into the Anaerobic Intrinsic Bioremediation of Whole Gasoline
R827015C018 Using Plants to Remediate Petroleum-Contaminated Soil
R827015C019 Biodegradation of Petroleum Hydrocarbons in Salt-Impacted Soil by Native Halophiles or Halotolerants and Strategies for Enhanced Degradation
R827015C020 Anaerobic Intrinsic Bioremediation of MTBE
R827015C021 Evaluation of Commercial, Microbial-Based Products to Treat Paraffin Deposition in Tank Bottoms and Oil Production Equipment
R827015C022 A Continuation: Humate-Induced Remediation of Petroleum Contaminated Surface Soils
R827015C023 Data for Design of Vapor Recovery Units for Crude Oil Stock Tank Emissions
R827015C024 Development of an Environmentally Friendly and Economical Process for Plugging Abandoned Wells
R827015C025 A Continuation of Remediation of Brine Spills with Hay
R827015C026 Identifying the Signature of the Natural Attenuation of MTBE in Goundwater Using Molecular Methods and "Bug Traps"
R827015C027 Identifying the Signature of Natural Attenuation in the Microbial
Ecology of Hydrocarbon Contaminated Groundwater Using Molecular Methods and
"Bug Traps"
R827015C028 Using Plants to Remediate Petroleum-Contaminated Soil: Project Continuation
R827015C030 Effective Stormwater and Sediment Control During Pipeline Construction Using a New Filter Fence Concept
R827015C031 Evaluation of Sub-micellar Synthetic Surfactants versus Biosurfactants for Enhanced LNAPL Recovery
R827015C032 Utilization of the Carbon and Hydrogen Isotopic Composition of Individual Compounds in Refined Hydrocarbon Products To Monitor Their Fate in the Environment
R830633 Integrated Petroleum Environmental Consortium (IPEC)
R830633C001 Development of an Environmentally Friendly and Economical Process for Plugging Abandoned Wells (Phase II)
R830633C002 A Continuation of Remediation of Brine Spills with Hay
R830633C003 Effective Stormwater and Sediment Control During Pipeline Construction Using a New Filter Fence Concept
R830633C004 Evaluation of Sub-micellar Synthetic Surfactants versus Biosurfactants for Enhanced LNAPL Recovery
R830633C005 Utilization of the Carbon and Hydrogen Isotopic Composition of Individual Compounds in Refined Hydrocarbon Products To Monitor Their Fate in the Environment
R830633C006 Evaluation of Commercial, Microbial-Based Products to Treat Paraffin Deposition in Tank Bottoms and Oil Production Equipment
R830633C007 Identifying the Signature of the Natural Attenuation in the Microbial Ecology of Hydrocarbon Contaminated Groundwater Using Molecular Methods and “Bug Traps”
R830633C008 Using Plants to Remediate Petroleum-Contaminated Soil: Project Continuation
R830633C009 Use of Earthworms to Accelerate the Restoration of Oil and Brine Impacted Sites
The perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Conclusions drawn by the principal investigators have not been reviewed by the Agency.
Project Research Results
1 journal articles for this subproject
Main Center: R827015
120 publications for this center
16 journal articles for this center