An in vivo Model for Detection of Reproductive Effects of Endocrine Disruptors

EPA Grant Number: R825298
Title: An in vivo Model for Detection of Reproductive Effects of Endocrine Disruptors
Investigators: Hinton, David E. , Teh, Swee J. , Washburn, Barbara S.
Institution: University of California - Davis , The University of Texas at El Paso
EPA Project Officer: Aja, Hayley
Project Period: November 1, 1996 through October 31, 1999
Project Amount: $519,729
RFA: Endocrine Disruptors (1996) RFA Text |  Recipients Lists
Research Category: Environmental Justice , Endocrine Disruptors , Human Health , Safer Chemicals


The objective of this research is to develop and validate a short term in vivo model, using the small teleost fish medaka, Oryzias latipes, to identify adverse effects of exposure to endocrine-disrupting chemicals. Reproductive endocrine dysfunction at the individual level will be emphasized. The most sensitive developmental window for exposure to endocrine disruptors (ED) will be identified using estradiol or androgen. Subsequently, appropriate exposure levels for two test compounds, octylphenol (OP), a xenoestrogen, and vinclozolin (V), an anti-androgen, will be determined. Effects on reproduction, will include mating behavior, sperm number and motility, egg number and quality, sex ratio, and liver and gonadal histology. Physiological and pathological changes in the liver and gonads will be correlated with reproductive success, embryo survival, and viability of fry. Effects of long term ED exposure outside the window of sensitivity will also be studied. Eggs produced by crossing exposed and unexposed female and male fish will be incubated and the development and reproductive success of the fry followed (P1 generation). To further validate the model, different EDs with known but different mechanisms of action will be investigated during the most sensitive development stage. If adverse effects are associated with exposure, further study will be performed.

Publications and Presentations:

Publications have been submitted on this project: View all 9 publications for this project

Journal Articles:

Journal Articles have been submitted on this project: View all 6 journal articles for this project

Supplemental Keywords:

endocrine disruption, fish, test, animal model, xenoestrogen, anit-androgen, reproduction, male, female, RFA, Health, Scientific Discipline, Environmental Chemistry, Health Risk Assessment, Endocrine Disruptors - Environmental Exposure & Risk, endocrine disruptors, Biochemistry, Children's Health, Biology, Endocrine Disruptors - Human Health, adverse outcomes, male, embryo survival, fish, anti-androgen, endocrine disrupting chemicals, in vivo model, animal models, developmental processes, xenoestrogen, reproductive processes, biological effects

Progress and Final Reports:

  • 1997
  • 1998
  • Final Report