Examination of the Estrogen Response Pathways in a New Vertebrate Model

EPA Grant Number: R825297
Title: Examination of the Estrogen Response Pathways in a New Vertebrate Model
Investigators: Linney, Elwood
Institution: Duke University Medical Center
EPA Project Officer: Aja, Hayley
Project Period: November 11, 1996 through November 10, 1999
Project Amount: $209,311
RFA: Endocrine Disruptors (1996) RFA Text |  Recipients Lists
Research Category: Endocrine Disruptors , Human Health , Safer Chemicals


The purpose of this project is to investigate the estrogen response in zebrafish with particular attention paid to embryos and young fish. One means will be to investigate the expression of the zebrafish estrogen receptor during these times using whole mount in situ hybridization. Some of this will be done with fluorescence so that we can three-dimensionally reconstruct and localize the estrogen receptor mRNA during embryogenesis and to also examine its expression under the influence of embryo treatment with estrogens. A second approach will involve the examination of expression from an introduce estrogen responsive reporter gene that provides a localized fluorescent signal (the green fluorescent protein gene). With this reporter we hope to record gene expression within the whole organism and capture this using the laser scanning confocal microscope.

Publications and Presentations:

Publications have been submitted on this project: View all 1 publications for this project

Supplemental Keywords:

RFA, Health, Scientific Discipline, Health Risk Assessment, Environmental Chemistry, Endocrine Disruptors - Environmental Exposure & Risk, endocrine disruptors, Biochemistry, Children's Health, Molecular Biology/Genetics, Biology, Endocrine Disruptors - Human Health, adverse outcomes, mRNA, fish, laser scanning microscope, endocrine disrupting chemicals, exposure studies, embryogenesis, animal models, physiology, estrogen response, screening methods, biological effects, estrogen receptors

Progress and Final Reports:

  • 1997
  • 1998
  • Final Report