Grantee Research Project Results
Development of an Immunobiosensor for Detection of PCBs
EPA Contract Number: 68D60022Title: Development of an Immunobiosensor for Detection of PCBs
Investigators: Ray, Bryan L.
Small Business: SymBiotech Inc.
EPA Contact: Richards, April
Phase: I
Project Period: September 1, 1996 through March 1, 1997
Project Amount: $70,000
RFA: Small Business Innovation Research (SBIR) - Phase I (1996) RFA Text | Recipients Lists
Research Category: Ecological Indicators/Assessment/Restoration , SBIR - Monitoring , Small Business Innovation Research (SBIR)
Description:
This proposal aims to develop an immuno-based biosensor for detecting PCBs in soil at 50ng/g and in water at 1 ng/L when combined with a sample concentration method to be developed in this proposal. The immunobiosensor will employ a photodetection system comprising bacteriorhodopsin, an integral membrane protein of the purple membrane of Halobacterium halobium. Bacteriorhodopsin pumps protons across the membrane when exposed to light. This response to light can be electronically monitored when purple membranes are deposited onto an electrode. Specific affinity interactions of antibodies with haptens (e.g., PCBs) can alter the photoresponse of these purple membranes. The ability of antibody/hapten inter-actions to quantitatively alter this photoresponse forms the basis for the immunobiosensor technology. The feasability of this technology will be determined.Supplemental Keywords:
small business, SBIR, engineering, chemistry, immunoassay, hazardous waste, RFA, Ecosystem Protection/Environmental Exposure & Risk, Scientific Discipline, Water, Waste, Toxics, Hazardous, pesticides, Environmental Chemistry, Contaminated Sediments, Monitoring/Modeling, Hazardous Waste, Environmental Engineering, Environmental Monitoring, bioassay, biosensors, membrane technology, pesticide residue, soil, biosensor, immunobiosensor, monitoring, soil sediment, contaminated soil, PCB, PCB detoxification, immunobisensor, biosensing, analytical chemistry, biosensing system, polychlorinated biphenyls (PCBs), hazardous chemicalsProgress and Final Reports:
The perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Conclusions drawn by the principal investigators have not been reviewed by the Agency.