Design and Synthesis of CO2-Soluble Affinity Ligands for Use in CO2 Extraction of Proteins

EPA Grant Number: R824730
Title: Design and Synthesis of CO2-Soluble Affinity Ligands for Use in CO2 Extraction of Proteins
Investigators: Beckman, Eric J. , Russell, Alan J.
Institution: University of Pittsburgh
EPA Project Officer: Hahn, Intaek
Project Period: October 1, 1995 through September 30, 1998
Project Amount: $150,000
RFA: Technology for a Sustainable Environment (1995) Recipients Lists
Research Category: Nanotechnology , Sustainability , Pollution Prevention/Sustainable Development


This proposal involves the design of highly CO2-soluble ligands for the extraction of molecules of biological origin. Carbon dioxide is a relatively benign solvent, and one where recovery of solutes from solution is easily accomplished via depressurization. Unfortunately, CO2 is a low dielectric fluid, and thus will not solubilize polar/hydrophilic molecules such as are commonly generated in biotechnical processes. Further, CO2 is a poor solvent for many commonly used extractant molecules, such as sufactants and chelating agents. Thus, this proposal focuses on the design of extractants/ligands particularly for use in CO2, to allow extension of CO2 to processes where its use is currently untenable. This is a jointly funded NSF/EPA project. In the EPA part of the project, we are focusing on the design of CO2-soluble chiral mobile phase agents (CMPA's) for the separation of enantiomers of ibuprofen and naproxen. A well-designed CMPA interacts specifically with one enantiomer of a racemic mixture, and thus allows separation, often times via salt formation and thus precipitation. Our goal is to combine the selectivity of a CMPA with the pressure-sensitive phase behavior inherent to CO2-based solutions to increase selectivity in a CMPA-moderated separation.

Publications and Presentations:

Publications have been submitted on this project: View all 11 publications for this project

Journal Articles:

Journal Articles have been submitted on this project: View all 2 journal articles for this project

Supplemental Keywords:

Carbon-dioxide-soluble affinity ligands; biological extraction; separation of enantiomer; racemic target molecules; chiral mobile phase ligands; carbon-dioxide-philic tails; fluoroalkyl and fluoroether., RFA, Scientific Discipline, Sustainable Industry/Business, cleaner production/pollution prevention, Environmental Chemistry, Sustainable Environment, Technology for Sustainable Environment, Ecological Risk Assessment, bilogical extraction, cleaner production, environmentally benign solvents, depressurization, carbon dioxide extraction, proteins, ligands, chiral mobile phase agents

Progress and Final Reports:

  • 1996
  • 1997
  • Final Report