Final Report: BioWinol Technologies: A Hybrid Green Process for Biofuel Production

EPA Grant Number: SU834728
Title: BioWinol Technologies: A Hybrid Green Process for Biofuel Production
Investigators: Huhnke, Raymond , Dharman, Karthikeyan Ramachandriya , Krishnan, Divya , Kundiyana, Dimple , Megel, Anthony , Terrill, Jenny , Wilkins, Mark
Institution: Oklahoma State University
EPA Project Officer: Nolt-Helms, Cynthia
Phase: I
Project Period: August 15, 2010 through August 14, 2011
Project Amount: $10,000
RFA: P3 Awards: A National Student Design Competition for Sustainability Focusing on People, Prosperity and the Planet (2010) RFA Text |  Recipients Lists
Research Category: Pollution Prevention/Sustainable Development , P3 Challenge Area - Energy , P3 Awards , Sustainability


BioWinol Technologies proposes a unique hybrid technology that uses several renewable resources, specifically biomass, wind and solar, to produce hydrogen (H2). This process can also capture carbon-dioxide (CO2) from other industries to produce biofuels. The primary objective for this Phase I project is to perform laboratory studies to determine the ability of two unique strains of bacteria to effectively utilize H2 and CO2 to produce biofuels. This objective was achieved by small bottle laboratory experiments to test and evaluate the effects of gas and media composition on two unique bacteria to produce ethanol. Three statistically designed experiments were conducted to screen and optimize the bacterial strain, feed gas composition and fermentation media components.

Summary/Accomplishments (Outputs/Outcomes):

Results indicate that bacteria Clostridium carboxidivorans (P7) gave comparatively higher ethanol concentration compared to Clostridium ragsdalei (P11). Other value added products such as acetic acid and butanol were also produced. Concentration of these products was higher with C. ragsdalei than with C. carboxidivorans. Maximum concentrations of ethanol, acetic acid and butanol from different fermentation treatments, averaged 2.78 gL-1, 4.06 gL-1 and 0.70 gL-1 respectively. These results are comparable to the products being formed in traditional syngas fermentations.


Acetogenic bacteria such as C. carboxidivorans and C. ragsdalei are able to ferment different combinations of CO2 and H2 to produce renewable fuel such as ethanol. An important path forward for a commercial facility is to scale-up the results from the laboratory studies. Scale-up studies in bench-scale and pilot-scale have been planned to be conducted in the last quarter of Phase I and in Phase II.

Supplemental Keywords:

Biofuel, Ethanol, Fermentation, Acetogen, Wind, Carbon dioxide

Relevant Websites:

BioWinol - Oklahoma State University Exit
OSU Biobased Products and Energy Center Exit
Oklahoma State University BioWinol Project Seeking Public Input Exit

P3 Phase II:

BioWinol Technologies: A hybrid green process for biofuel production – Phase 2