Aerobic Cometabolism of Chlorinated Ethenes by Microorganisms that Grow on Organic Acids and Alcohols

EPA Grant Number: R828772C010
Subproject: this is subproject number 010 , established and managed by the Center Director under grant R828772
(EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).

Center: HSRC (2001) - Western Region Hazardous Substance Research Center for Developing In-Situ Processes for VOC Remediation in Groundwater and Soils
Center Director: Semprini, Lewis
Title: Aerobic Cometabolism of Chlorinated Ethenes by Microorganisms that Grow on Organic Acids and Alcohols
Investigators: Bottomley, Peter , Arp, Daniel J. , Dolan, Mark E. , Semprini, Lewis
Institution: Oregon State University
EPA Project Officer: Lasat, Mitch
Project Period: September 1, 2001 through August 31, 2006
RFA: Hazardous Substance Research Centers - HSRC (2001) RFA Text |  Recipients Lists
Research Category: Land and Waste Management , Hazardous Waste/Remediation

Objective:

The Part I project aims to evaluate how to maximize the chloroethene degrading potential of individual strains and mixed communities of hydrocarbon-degrading bacteria. Specific subobjectives include identifying conditions that maximize reductant flow to cometabolism and that promote maximum expression of monooxygenase genes and enzyme activity.

A primary goal of the Part II project is to isolate and characterize pure cultures that can transform cis-dichloroethene (cis-DCE) and vinyl chloride (VC) when grown on acetate, propionate, and butyrate. Initially, the proposal was to attempt isolation of members of an enrichment culture, BA-1, known to co-oxidize cis-DCE when grown on organic acids. However, upon recommendation of the Science Advisory Committee (SAC), our initial focus has changed to isolation and metabolic evaluation of cultures able to directly metabolize cis-DCE and VC.

Publications and Presentations:

Publications have been submitted on this subproject: View all 18 publications for this subprojectView all 158 publications for this center

Journal Articles:

Journal Articles have been submitted on this subproject: View all 5 journal articles for this subprojectView all 60 journal articles for this center

Progress and Final Reports:

  • 2002
  • 2003
  • 2004 Progress Report
  • 2005 Progress Report
  • Final

  • Main Center Abstract and Reports:

    R828772    HSRC (2001) - Western Region Hazardous Substance Research Center for Developing In-Situ Processes for VOC Remediation in Groundwater and Soils

    Subprojects under this Center: (EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).
    R828772C001 Developing and Optimizing Biotransformation Kinetics for the Bio- remediation of Trichloroethylene at NAPL Source Zone Concentrations
    R828772C002 Strategies for Cost-Effective In-situ Mixing of Contaminants and Additives in Bioremediation
    R828772C003 Aerobic Cometabolism of Chlorinated Aliphatic Hydrocarbon Compounds with Butane-Grown Microorganisms
    R828772C004 Chemical, Physical, and Biological Processes at the Surface of Palladium Catalysts Under Groundwater Treatment Conditions
    R828772C005 Effects of Sorbent Microporosity on Multicomponent Fate and Transport in Contaminated Groundwater Aquifers
    R828772C006 Development of the Push-Pull Test to Monitor Bioaugmentation with Dehalogenating Cultures
    R828772C007 Development and Evaluation of Field Sensors for Monitoring Bioaugmentation with Anaerobic Dehalogenating Cultures for In-Situ Treatment of TCE
    R828772C008 Training and Technology Transfer
    R828772C009 Technical Outreach Services for Communities (TOSC) and Technical Assistance to Brownfields Communities (TAB) Programs
    R828772C010 Aerobic Cometabolism of Chlorinated Ethenes by Microorganisms that Grow on Organic Acids and Alcohols
    R828772C011 Development and Evaluation of Field Sensors for Monitoring Anaerobic Dehalogenation after Bioaugmentation for In Situ Treatment of PCE and TCE
    R828772C012 Continuous-Flow Column Studies of Reductive Dehalogenation with Two Different Enriched Cultures: Kinetics, Inhibition, and Monitoring of Microbial Activity
    R828772C013 Novel Methods for Laboratory Measurement of Transverse Dispersion in Porous Media
    R828772C014 The Role of Micropore Structure in Contaminant Sorption and Desorption