Genomic Database for Cryptosporidium spp.

EPA Grant Number: R825148
Title: Genomic Database for Cryptosporidium spp.
Investigators: Upton, Steve J.
Current Investigators: Upton, Steve J. , Blagburn, Byron L. , Dykstra, Christine C.
Institution: Kansas State University
Current Institution: Kansas State University , Auburn University Main Campus
EPA Project Officer: Hiscock, Michael
Project Period: September 23, 1996 through September 22, 1999 (Extended to September 22, 2000)
Project Amount: $304,755
RFA: Drinking Water (1996) RFA Text |  Recipients Lists
Research Category: Drinking Water , Water


Using molecular techniques, we plan to construct gDNA libraries for various isolates and species of Cryptosporidium. In total, about 12 isolates of C. parvum and perhaps 8-10 other species of the genus will be included. These libraries will be made accessible through the American Type Culture Collection for general distribution to researchers. These libraries should be useful to investigators who plan to design genetic probes for environmental testing. The advantage of these libraries will be to: 1) allow investigators to sequence and study any region of the genome of their choice without having to rely on previously published sequences from other researchers; 2) allow investigators to develop specific, rather than random, primers for developing PCR based diagnostic tests; and 3) to archive the DNA of various isolates and species in a manner where information can be retrieved in perpetuity.

Publications and Presentations:

Publications have been submitted on this project: View all 13 publications for this project

Journal Articles:

Journal Articles have been submitted on this project: View all 9 journal articles for this project

Supplemental Keywords:

pathogens, drinking water, genetics, Kansas, human health, environmental testing, exposure., RFA, Scientific Discipline, Water, Environmental Chemistry, Genetics, Health Risk Assessment, Analytical Chemistry, Drinking Water, pathogens, public water systems, Safe Drinking Water, monitoring, microbial risk assessment, detection, human health effects, exposure and effects, exposure, analytical methods, cryptosporidium , genomic database, public health, treatment, microbial risk management, emerging pathogens, DNA libraries, drinking water contaminants, infectivity

Relevant Websites:

Multiple Web sites have been created either all, or in part, as a component of this grant. These sites, which reside at Kansas State University, include the following: (this Web site provides a selective bibliography on a variety of Cryptosporidium topics). (this Web site provides a list of commercially available reagents and genomic libraries for Cryptosporidium research). (this Web site provides a list of the various isolates for which we have accumulated oocyst measurements). (this Web site provides a chronological (historical) listing of the taxonomic milestones for the genus Cryptosporidium). (this Web site provides a list of select references concerning waterborne/epidemiology of Cryptosporidium).

Progress and Final Reports:

  • 1997
  • 1998
  • 1999
  • Final Report