Rapid and Quantitative Detection of Helicobacter Pylori and E. Coli O157 in Well Water Using a Nano-Wired Biosensor and QPCR

EPA Grant Number: R833005
Title: Rapid and Quantitative Detection of Helicobacter Pylori and E. Coli O157 in Well Water Using a Nano-Wired Biosensor and QPCR
Investigators: Alocilja, Evangelyn C. , Molloy, Stephanie L. , Rose, Joan B.
Current Investigators: Alocilja, Evangelyn C. , Dreelin, Erin , Rose, Joan B.
Institution: Michigan State University
EPA Project Officer: Hiscock, Michael
Project Period: November 1, 2006 through October 31, 2009 (Extended to October 31, 2011)
Project Amount: $600,000
RFA: Development and Evaluation of Innovative Approaches for the Quantitative Assessment of Pathogens in Drinking Water (2005) RFA Text |  Recipients Lists
Research Category: Drinking Water , Water

Objective:

The hypothesis of this proposal is that a disposable biosensor and QPCR can be combined seamlessly to develop a unique biosensor-QPCR as a tool for near real-time determination of contaminant occurrence in drinking water. The specific objectives are: (1) Develop a protocol for processing water samples for the biosensor and QPCR; (2) Assess the performance of the biosensor and QPCR for sensitivity, specificity, recovery, and false positives/negatives of detection and enumeration for E. coli O157:H7 and H. pylori in groundwater samples from the field; (3) Develop a method for detecting and enumerating E. coli O157:H7 and H. pylori by QPCR using bacteria isolated and screened by the biosensor system; and (4) Validate a method for testing viability of E. coli O157:H7.

Approach:

The first step in our experimental approach is to evaluate the performance of the biosensor and QPCR separately. Once confirmed, a method to seamlessly integrate these two devices into a biosensor-to-QPCR field-lab technique, coupled with a viability test, will be developed.

Expected Results:

This project is expected to advance the use of antibody-based methods and molecular techniques for application to drinking water supplies. The expected deliverables are: (a) proof of concept and assessment of biosensor and QPCR techniques for recovery, detection and quantitation of bacteria in water; and (b) the development of a unique attribute that is not in the literature at the moment: the capability for direct confirmation through QPCR of presumptive results from the biosensor, thereby minimizing false positive and false negative analysis.

Publications and Presentations:

Publications have been submitted on this project: View all 11 publications for this project

Journal Articles:

Journal Articles have been submitted on this project: View all 3 journal articles for this project

Supplemental Keywords:

Field-based detection, biosensor-QPCR technique, drinking water, pathogens, innovative technology,, RFA, Scientific Discipline, INTERNATIONAL COOPERATION, Water, TREATMENT/CONTROL, POLLUTANTS/TOXICS, Sustainable Industry/Business, Sustainable Environment, Technology, Technology for Sustainable Environment, Environmental Monitoring, Drinking Water, Environmental Engineering, Microorganisms, pathogens, bacteria, nanotechnology, drinking water monitoring, E. Coli, biotechnology, biosensors, biosensor

Progress and Final Reports:

  • 2007 Progress Report
  • 2008 Progress Report
  • 2009
  • 2010
  • Final