Molecular Detection and Environmental Survey of Vegetative and Coccoid Helicobacter pyloriEPA Grant Number: R828037
Title: Molecular Detection and Environmental Survey of Vegetative and Coccoid Helicobacter pylori
Investigators: Shahamat, Manouchehr , Bradley, Brian , Levin, M. , Robb, Frank , Sowers, Kevin
Current Investigators: Shahamat, Manouchehr , Alavi, Mohammad , Bradley, Brian , Gonzalez, Juan M , Hind, John , Maeder, Dennis , Pavlick, Christine , Robb, Frank , Sowers, Kevin , Watts, Joy
Institution: University of Maryland Biotechnology Institute
EPA Project Officer: Nolt-Helms, Cynthia
Project Period: May 15, 2000 through May 14, 2003
Project Amount: $522,145
RFA: Drinking Water (1999) RFA Text | Recipients Lists
Research Category: Drinking Water , Water
The basic goals of this research proposal are to validate an accurate and sensitive molecular and/or immunodiagnostic method for rapid detection of H. pylori in both the culturable and in coccoid (i.e., VNC, viable, not culturable) states in environmental samples and examine the relationship between H. pylori and indicators of fecal pollution. This ability is crucial for preventing underestimation of the number of bacteria in samples, for developing epidemiological evidence, and for understanding the ecological and public health significance.
Phase 1: Immunological assay
- Produce monoclonal antibodies specific to the helical and coccoid form of H. pylori.
- Optimize a PCR based method using highly specific oligonucleotide probes for detection of vegetative as well as coccoid forms in environmental samples.
- Optimize and validate an immunodiagnostic protocol for rapid detection of the coccoid form in environmental samples.
- Examine the relationship between H. pylori and indicators of fecal contamination and initiate studies to identify the habitat of the organism in environmental samples.
We propose to: (1) produce a monoclonal antibody specific to H. pylori and develop an immunodiagnostic method for rapid detection of the bacterium in coccoid form; (2) develop highly specific PCR-based method for detection of H. pylori in the vegetative and coccoid forms in clinical and environmental samples; and (3) develop an oligonucleotide probe for detection of H. pylori in environmental samples, especially in coccoid form.