2006 Progress Report: Air Pollution, Exhaled Breath Markers, and Asthma in Susceptible Children

EPA Grant Number: R831861C003
Subproject: this is subproject number 003 , established and managed by the Center Director under grant R831861
(EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).

Center: USC Center for Children’s Environmental Health
Center Director: Gilliland, Frank D.
Title: Air Pollution, Exhaled Breath Markers, and Asthma in Susceptible Children
Investigators: Gilliland, Frank D.
Institution: University of Southern California
EPA Project Officer: Callan, Richard
Project Period: November 1, 2003 through October 31, 2008 (Extended to October 31, 2010)
Project Period Covered by this Report: November 1, 2005 through October 31, 2006
RFA: Centers for Children's Environmental Health and Disease Prevention Research (2003) RFA Text |  Recipients Lists
Research Category: Health Effects , Children's Health , Health

Objective:

In this project, we proposed to assess the following hypotheses:
Hypothesis 1 (H1): High ambient air pollution exposure is associated with chronic airway inflammation in children as indicated by elevated eNO, a marker of airway inflammation and oxidative/nitrosative stress.
Hypothesis 2 (H2): Children's susceptibility to airway inflammation and oxidative/nitrosative stress from ambient air pollution varies by NOS1, NOS2 and NOS3, GSTM1, GSTP1, NQO1, and HO-1 genotypes.
Hypothesis 3 (H3): Children with chronic airway inflammation as indicated by elevated eNO are at increased risk for new onset asthma.

We will assess our hypotheses by accomplishing the following specific aims:
Specific Aim 1 (SA1): Collect eNO from 3000 children in the ongoing AIR study cohort.
Specific Aim 2 (SA2): Genotype the cohort of 3000 children for functional polymorphism/haplotypes in the NOS1, NOS2 and NOS3, GSTM1, GSTP1, NQO1, and HO-1.
Specific Aim 3 (SA3): To use a multilevel design to determine the relationship between levels of eNO with short- and long-term air pollution exposures and to assess the effects of genetic variation in NOS1, 2, and 3 on these relationships using data collected in SA1, SA2 and air pollution exposure estimates from the ongoing cohort study.
Specific Aim 4 (SA4): To determine the risk for new onset asthma in children with high levels of eNO using data collected in SA1 and in the ongoing prospective cohort study of incident asthma.

Progress Summary:

In this period, we have developed protocols for field collection, tested methods, and trained filed staff. We have completed fieldwork for enrolling subjects in the study and collecting eNO. We are beginning analysis of the data. Buccal cells for DNA was collected, processed and stored in the Molecular Biology Core. We developed NOS assays and are beginning to extract DNA in preparation for genotyping. We also continue to follow the cohort for asthma status and exposure variables.

Exhaled NO (eNO) has the potential for use in understanding asthma etiology, for identifying high-risk children and for use as a marker of response in intervention studies. This program of innovative research builds on the results from studies in the initial five years of the Children’s Environmental Health Center, the population resource of a large ongoing prospective cohort study of the determinants of childhood asthma incidence and an extensive cutting edge air pollution exposure assessment program to efficiently fill key research and public health needs.

Future Activities:

We plan to continue to analyzing the eNO data, complete DNA extraction and begin genotyping the cohort for the proposed genes, and continued developing exposure metrics as the exposure measurements become available. We plan to investigate the use or GIS methods to examine these data. We plan to submit a manuscript on the determinants of eNO is this large population-based sample as our first paper.


Journal Articles on this Report : 12 Displayed | Download in RIS Format

Other subproject views: All 141 publications 91 publications in selected types All 90 journal articles
Other center views: All 193 publications 125 publications in selected types All 124 journal articles
Type Citation Sub Project Document Sources
Journal Article Gauderman WJ, Avol E, Lurmann F, Kuenzli N, Gilliland F, Peters J, McConnell R. Childhood asthma and exposure to traffic and nitrogen dioxide. Epidemiology 2005;16(6):737-743. R831861 (2004)
R831861 (2005)
R831861 (2006)
R831861 (Final)
R831861C001 (2005)
R831861C001 (2006)
R831861C001 (Final)
R831861C002 (Final)
R831861C003 (2006)
R831861C003 (Final)
R827352 (Final)
R827352C007 (Final)
R827352C009 (Final)
  • Abstract from PubMed
  • Full-text: Mothers for Clean Air Colorado-Full Text PDF
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  • Abstract: Epidemiology-Abstract
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  • Journal Article Gilliland F, Avol E, Kinney P, Jerrett M, Dvonch T, Lurmann F, Buckley T, Breysse P, Keeler G, de Villiers T, McConnell R. Air pollution exposure assessment for epidemiologic studies of pregnant women and children: lessons learned from the Centers for Children's Environmental Health and Disease Prevention Research. Environmental Health Perspectives 2005;113(10):1447-1454. R831861 (2004)
    R831861 (2005)
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    R831861C001 (2006)
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    R826708 (2000)
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    R826710 (Final)
    R827027 (2002)
    R831845 (2005)
    R832139 (2006)
    R832141 (2006)
    R832141 (2007)
    R832141 (Final)
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  • Journal Article Li Y-F, Langholz B, Salam MT, Gilliland FD. Maternal and grandmaternal smoking patterns are associated with early childhood asthma. Chest 2005;127(4):1232-1241. R831861 (2004)
    R831861 (2005)
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    R831861 (Final)
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    R826708 (2000)
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  • Abstract from PubMed
  • Full-text: Chest-Full Text HTML
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  • Abstract: Chest-Abstract
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  • Other: Chest-Full Text PDF
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  • Journal Article Li Y-F, Tsao YH, Gauderman WJ, Conti DV, Avol E, Dubeau L, Gilliland FD. Intercellular adhesion molecule-1 and childhood asthma. Human Genetics 2005;117(5):476-484. R831861 (2004)
    R831861 (2005)
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    R831861 (Final)
    R831861C001 (Final)
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    R826708 (2000)
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  • Abstract: SpringerLink-Abstract
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  • Journal Article Li Y-F, Gauderman WJ, Avol E, Dubeau L, Gilliland FD. Associations of tumor necrosis factor G-308A with childhood asthma and wheezing. American Journal of Respiratory and Critical Care Medicine 2006;173(9):970-976. R831861 (2004)
    R831861 (2005)
    R831861 (2006)
    R831861 (Final)
    R831861C001 (Final)
    R831861C002 (Final)
    R831861C003 (2006)
    R831861C003 (Final)
    R826708 (2000)
    R826708 (2001)
    R826708 (2002)
    R826708 (Final)
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  • Full-text: ATS-Full Text HTML
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  • Abstract: ATS-Abstract
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  • Other: ATS-Full Text PDF
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  • Journal Article McConnell R, Berhane K, Yao L, Jerrett M, Lurmann F, Gilliland F, Kunzli N, Gauderman J, Avol E, Thomas D, Peters J. Traffic, susceptibility, and childhood asthma. Environmental Health Perspectives 2006;114(5):766-772. R831861 (2004)
    R831861 (2005)
    R831861 (2006)
    R831861 (Final)
    R831861C001 (2006)
    R831861C001 (Final)
    R831861C002 (Final)
    R831861C003 (2006)
    R831861C003 (2007)
    R831861C003 (Final)
    R826708 (Final)
    R827352 (Final)
    R827352C007 (Final)
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  • Abstract from PubMed
  • Associated PubMed link
  • Journal Article Millstein J, Gilliland F, Berhane K, Gauderman WJ, McConnell R, Avol E, Rappaport EB, Peters JM. Effects of ambient air pollutants on asthma medication use and wheezing among fourth-grade school children from 12 Southern California communities enrolled in The Children's Health Study. Archives of Environmental Health 2004;59(10):505-514. R831861 (2006)
    R831861 (Final)
    R831861C001 (Final)
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    R831861C003 (2006)
    R831861C003 (Final)
  • Abstract from PubMed
  • Abstract: Taylor Francis Online-Abstract
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  • Journal Article Millstein J, Conti DV, Gilliland FD, Gauderman WJ. A testing framework for identifying susceptibility genes in the presence of epistasis. American Journal of Human Genetics 2006;78(1):15-27. R831861 (2004)
    R831861 (2005)
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  • Full-text: Science Direct-Full Text HTML
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  • Abstract: AJHG-Abstract
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  • Other: Science Direct-Full Text PDF
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  • Journal Article Salam MT, Li Y-F, Langholz B, Gilliland FD. Maternal fish consumption during pregnancy and risk of childhood asthma. Journal of Asthma 2005;42(6):513-518. R831861 (2004)
    R831861 (2005)
    R831861 (2006)
    R831861 (Final)
    R831861C001 (Final)
    R831861C002 (Final)
    R831861C003 (2006)
    R831861C003 (Final)
    R826708 (2000)
    R826708 (2001)
    R826708 (2002)
    R826708 (Final)
  • Abstract from PubMed
  • Abstract: Taylor and Francis-Abstract
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  • Journal Article Salam MT, Millstein J, Li YF, Lurmann FW, Margolis HG, Gilliland FD. Birth outcomes and prenatal exposure to ozone, carbon monoxide, and particulate matter: results from the Children's Health Study. Environment Health Perspectives 2005;113(11):1638-1644. R831861 (2004)
    R831861 (2005)
    R831861 (2006)
    R831861 (Final)
    R831861C001 (Final)
    R831861C002 (Final)
    R831861C003 (2006)
    R831861C003 (Final)
  • Full-text from PubMed
  • Abstract from PubMed
  • Associated PubMed link
  • Journal Article Salam MT, Margolis HG, McConnell R, McGregor JA, Avol EL, Gilliland FD. Mode of delivery is associated with asthma and allergy occurrences in children. Annals of Epidemiology 2006;16(5):341-346. R831861 (2004)
    R831861 (2005)
    R831861 (2006)
    R831861 (Final)
    R831861C001 (2006)
    R831861C001 (Final)
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    R831861C003 (2006)
    R831861C003 (Final)
  • Abstract from PubMed
  • Abstract: Annals of Epidemiology-Abstract
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  • Journal Article Wenten M, Berhane K, Rappaport EB, Avol E, Tsai WW, Gauderman WJ, McConnell R, Dubeau L, Gilliland FD. TNF-308 modifies the effect of second-hand smoke on respiratory illness-related school absences. American Journal of Respiratory and Critical Care Medicine 2005;172(12):1563-1568. R831861 (2004)
    R831861 (2005)
    R831861 (2006)
    R831861 (Final)
    R831861C001 (Final)
    R831861C002 (Final)
    R831861C003 (2006)
    R831861C003 (Final)
    R826708 (2000)
    R826708 (2001)
    R826708 (2002)
    R826708 (Final)
  • Full-text from PubMed
  • Abstract from PubMed
  • Associated PubMed link
  • Full-text: ATS Journals-Full Text PDF
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  • Abstract: ATS Journals-Abstract
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  • Supplemental Keywords:

    RFA, Health, Scientific Discipline, ENVIRONMENTAL MANAGEMENT, Health Risk Assessment, Children's Health, Risk Assessment, asthma, community-based intervention, airway disease, respiratory problems, Human Health Risk Assessment, childhood respiratory disease, susceptibility, children's environmental health, outreach and education

    Progress and Final Reports:

    Original Abstract
  • 2004 Progress Report
  • 2005 Progress Report
  • 2007 Progress Report
  • 2008
  • 2009
  • Final Report

  • Main Center Abstract and Reports:

    R831861    USC Center for Children’s Environmental Health

    Subprojects under this Center: (EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).
    R831861C001 Urban Air Pollution and Persistent Early Life Asthma
    R831861C002 Pollution-Enhanced Allergic Inflammation and Phase II Enzymes
    R831861C003 Air Pollution, Exhaled Breath Markers, and Asthma in Susceptible Children