Final Report: Integrated Microfluidic System for Bioluminescent Bioreporting, Separations, Vibrational Spectroscopy, and Microcantilever Transducer Evaluation of Endocrine Disrupting Chemicals

EPA Grant Number: R832740
Title: Integrated Microfluidic System for Bioluminescent Bioreporting, Separations, Vibrational Spectroscopy, and Microcantilever Transducer Evaluation of Endocrine Disrupting Chemicals
Investigators: Sepaniak, Michael J. , Sayler, Gary S.
Institution: University of Tennessee - Knoxville
EPA Project Officer: Hahn, Intaek
Project Period: November 1, 2005 through October 31, 2008 (Extended to October 31, 2009)
Project Amount: $590,240
RFA: Exposure Measurement Tools for Endocrine Disrupting Chemicals in Mixtures (2005) RFA Text |  Recipients Lists
Research Category: Economics and Decision Sciences , Health , Safer Chemicals , Endocrine Disruptors

Objective:

The objective of this research has been to develop analytical methodologies for the quantitative and qualitative measurement of mixtures of endocrine disrupting chemicals by utilizing the tools of analytical chemistry that include microcantilever arrays (MCA) for nanomechanical sensing, surface enhanced Raman spectroscopy (SERS), and chemical separations and solid phase concentration complimented by biosensing using microorganism receptor elements. By improving the existing technology and attacking the problem of EDC exposure and activity monitoring with an arsenal of informative tools such as MCAs, SERS, and chemical separations, one can clarify and elucidate which chemicals, and in what combinations, can mimic or inhibit endocrine signaling molecules. Speed and portability of these analytical techniques, either on separate or partially integrated platforms, could facilitate in field screening and characterization of samples.

Summary/Accomplishments (Outputs/Outcomes):

Task 1. With advanced substrates, test known EDCs in relevant matrices for SERS response characteristics.
Surveys of SERS responses of many EDCs have been made that are distinctive, but inadequate sensitivity and cost issues hindered success. We have addressed this with a novel approach to nanofabrication of high performing substrates and with integrating with µ-fluidics & on-line concentrators.
 
Task 2. Develop EDC nanomechanical response signatures on functionalized microcantilever arrays.
Nanomechnical sensing using MCAs with conventional (organic coatings) responsive phases lacked desired sensitivity and selectivity. This issue has been successfully addressed in the latter stages of the work with nuclear receptor proteins as selective and sensitive responsive phases that can probe many ligand (EDC) – receptor interactions.
 
Task 3. Integrate bioreporter elements and conditions onto µfluidic platforms with electrophoretic separations (including extraction/concentration) of EDCs.
Capillary electrophoretic separations of representative EDCs have been performed and coupled with fluorescence detection and SERS. A novel lab-on-a-chip platform was pursued in Year 3 and the extension. Bioreporting elements (receptor proteins such as ER and TR) have been included in MCA systems (see above).
 
Task 4. Validate integrated µfluidic devices that incorporates combinations of these analytical components for EDC-containing samples.
Integration has progressed relatively slowly do to the gradual development of tasks 1-3. However, reasonable progress has been made with integrating separations with SERS, bioreporter elements (receptor proteins) with nanomechanics on MCs, and integrating extraction/concentration with these techniques. In addition, extensive bioreporting validation studies have been conducted by the Co-PI’s group using luminescence bioreporters.


Journal Articles on this Report : 25 Displayed | Download in RIS Format

Other project views: All 53 publications 30 publications in selected types All 30 journal articles
Type Citation Project Document Sources
Journal Article Abu-Hatab NA, John JF, Oran JM, Sepaniak MJ. Multiplexed microfluidic surface-enhanced Raman spectroscopy. Applied Spectroscopy 2007;61(10):1116-1122. R832740 (2007)
R832740 (2008)
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  • Journal Article Abu-Hatab NA, Oran JM, Sepaniak MJ. Surface-enhanced Raman spectroscopy substrates created via electron beam lithography and nanotransfer printing. ACS Nano 2008;2(2):377-385. R832740 (2008)
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  • Journal Article Archibald R, Datskos P, Devault G, Lamberti V, Lavrik N, Noid D, Sepaniak M, Dutta P. Independent component analysis of nanomechanical responses of cantilever arrays. Analytica Chimica Acta 2007;584(1):101-105. R832740 (2006)
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  • Journal Article Bhandari D, Walworth MJ, Sepaniak MJ. Dual function surface-enhanced Raman active extractor for the detection of environmental contaminants. Applied Spectroscopy 2009;63(5):571-578. R832740 (2008)
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  • Journal Article Bhandari D, Wells SM, Retterer SD, Sepaniak MJ. Characterization and detection of uranyl ion sorption on silver surfaces using surface enhanced Raman spectroscopy. Analytical Chemistry 2009;81(19):8061-8067. R832740 (Final)
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  • Journal Article Bhandari D, Wells SM, Polemi A, Kravchenko II, Shuford KL, Sepaniak MJ. Stamping plasmonic nanoarrays on SERS-supporting platforms. Journal of Raman Spectroscopy 2011;42(11):1916-1924. R832740 (Final)
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  • Journal Article Chapman PJ, Vogt F, Dutta P, Datskos PG, Devault GL, Sepaniak MJ. Facile hyphenation of gas chromatography and a microcantilever array sensor for enhanced selectivity. Analytical Chemistry 2007;79(1):364-370. R832740 (2006)
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  • Journal Article Chapman PJ, Long Z, Datskos PG, Archibald R, Sepaniak MJ. Differentially ligand-functionalized microcantilever arrays for metal ion identification and sensing. Analytical Chemistry 2007;79(18):7062-7068. R832740 (2008)
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  • Journal Article Connatser RM, Cochran M, Harrison RJ, Sepaniak MJ. Analytical optimization of nanocomposite surface-enhanced Raman spectroscopy/scattering detection in microfluidic separation devices. Electrophoresis 2008;29(7):1441-1450. R832740 (2008)
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  • Journal Article Dutta P, Hill K, Datskos PG, Sepaniak MJ. Development of a nanomechanical biosensor for analysis of endocrine disrupting chemicals. Lab on a Chip 2007;7(9):1184-1191. R832740 (2007)
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  • Journal Article Hill KL, Dutta P, Long Z, Sepaniak MJ. Microcantilever-based nanomechanical studies of the orphan nuclear receptor pregnane X receptor-ligand interactions. Journal of Biomaterials and Nanobiotechnology 2011;2(2):133-142. R832740 (Final)
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  • Journal Article Hill K, Dutta P, Zareba A, Eldridge ML, Sepaniak MJ. Morphological and chemical optimization of microcantilever surfaces for thyroid system biosensing and beyond. Analytica Chimica Acta 2008;625(1):55-62. R832740 (2007)
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  • Journal Article Lavrik NV, Taylor LC, Sepaniak MJ. Enclosed pillar arrays integrated on a fluidic platform for on-chip separations and analysis. Lab on a Chip 2010;10(8):1086-1094. R832740 (Final)
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  • Journal Article Lavrik NV, Taylor LT, Sepaniak MJ. Nanotechnology and chip level systems for pressure driven liquid chromatography and emerging analytical separation techniques: a review. Analytica Chimica Acta 2011;694(1-2):6-20. R832740 (Final)
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  • Journal Article Long Z, Storey J, Lewis S, Sepaniak MJ. Landfill siloxane gas sensing using differentiating, responsive phase coated microcantilever arrays. Analytical Chemistry 2009;81(7):2575-2580. R832740 (Final)
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  • Journal Article Long Z, Hill K, Sepaniak MJ. Aluminium oxide nanostructured microcantilever arrays for nanomechanical-based sensing. Analytical Chemistry 2010;82(10):4114-4121. R832740 (Final)
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  • Journal Article Olavarria-Fullerton J, Wells S, Ortiz-Rivera W, Sepaniak MJ, De Jesus MA. Surface-Enhanced Raman Scattering (SERS) characterization of trace organoarsenic antimicrobials using silver/polydimethylsiloxane nanocomposites. Applied Spectroscopy 2011;65(4):423-428. R832740 (Final)
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  • Journal Article Olavarria-Fullerton J, Velez RA, Wells S, Sepaniak MJ, Hernandez-Rivera SP, De Jesus MA. Design and characterization of hybrid morphology nanoarrays as plasmonic Raman probes for antimicrobial detection. Applied Spectroscopy 2013;67(11):1315-1322. R832740 (Final)
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  • Journal Article Oran JM, Hinde RJ, Abu-Hatab NA, Retterer ST, Sepaniak MJ. Nanofabricated periodic arrays of silver elliptical discs as SERS substrates. Journal of Raman Spectroscopy 2008;39(12):1811-1820. R832740 (2007)
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  • Journal Article Sanseverino J, Eldridge ML, Layton AC, Easter JP, Yarbrough J, Schultz TW, Sayler GS. Screening of potentially hormonally active chemicals using bioluminescent yeast bioreporters. Toxicological Sciences 2009;107(1):122-134. R832740 (2008)
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  • Journal Article Strong LL, Thompson B, Koepsell TD, Meischke H. Factors associated with pesticide safety practices in farmworkers. American Journal of Industrial Medicine 2008;51(1):69-81. R832740 (Final)
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  • Journal Article Walworth MJ, Connatser RM, Sepaniak MJ. Extraction, separation, and fluorometric analysis of selected environmental contaminants. Journal of Separation Science 2009;32(17):2985-2992. R832740 (Final)
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  • Journal Article Wellman AD, Sepaniak MJ. Magnetically-assisted transport evanescent field fluoroimmunoassay. Analytical Chemistry 2006;78(13):4450-4456. R832740 (2006)
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  • Journal Article Wellman AD, Sepaniak MJ. Multiplexed, waveguide approach to magnetically assisted transport evanescent field fluoroassays. Analytical Chemistry 2007;79(17):6622-6628. R832740 (2007)
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  • Journal Article Wells SM, Retterer SD, Oran JM, Sepaniak MJ. Controllable nanofabrication of aggregate-like nanoparticle substrates and evaluation for surface-enhanced Raman spectroscopy. ACS Nano 2009;3(12):3845-3853. R832740 (Final)
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  • Supplemental Keywords:

    MEMS, preconcentration, nuclear receptor protein sensing, bioreporters, microcantilever arrays, SPE-SERS, lab-on-a- chip
    , RFA, Scientific Discipline, Health, Health Risk Assessment, Endocrine Disruptors - Environmental Exposure & Risk, Microbiology, endocrine disruptors, Biochemistry, Endocrine Disruptors - Human Health, endocrine disruptor screening program, microcantilever transducer evaluation, bioavailability, bioluminescent testing, EDCs, endocrine disrupting chemicals, exposure studies

    Progress and Final Reports:

    Original Abstract
  • 2006 Progress Report
  • 2007 Progress Report
  • 2008 Progress Report