2009 Progress Report: Characterization and Source ApportionmentEPA Grant Number: R832415C001
Subproject: this is subproject number 001 , established and managed by the Center Director under grant R832415
(EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).
Center: Rochester PM Center
Center Director: Oberdörster, Günter
Title: Characterization and Source Apportionment
Investigators: Hopke, Philip K. , Gelein, Robert , Prather, Kimberly A.
Institution: Clarkson University , University of California - San Diego , University of Rochester
EPA Project Officer: Chung, Serena
Project Period: October 1, 2005 through September 30, 2010 (Extended to September 30, 2012)
Project Period Covered by this Report: October 1, 2008 through September 30,2009
RFA: Particulate Matter Research Centers (2004) RFA Text | Recipients Lists
Research Category: Health Effects , Air
Future Activities:During the next year, we will continue to use spin traps to explore the nature of the initially formed free radical species that form an important part of the reactive oxygen species associated with freshly formed secondary organic aerosols. We are also making ambient measurements using the spin traps in urban and rural environments to explore the presence of particle-bound free radical species. We are also exploring the potential use of new fluorogenic probes for the ROS measurement. Recently, Fairfull-Smith and Bottle (2008) have reported a series of new probes that appear to be more sensitive and with less blank problems than the dichlorofluorescin-based system we are currently using. We are working with Prof. Bottle to test his probes as we did with the other currently used probes (Venkatachari and Hopke, 2008a). They claim that their probes will be as sensitive, but with lower and more consistent blank values and without the self-oxidation seen with DCFH. The first batch of the probe is to be sent from the Queensland University of Technology to permit the initial experiments to be performed.
References:Fairfull-Smith, K.E., Bottle, S.E., (2008) The Synthesis and Physical Properties of Novel Polyaromatic Profluorescent Isoindoline Nitroxide Probes, European Journal of Organic Chemistry 2008 (32), 5391-5400.
Journal Articles on this Report : 1 Displayed | Download in RIS Format
|Other subproject views:||All 19 publications||13 publications in selected types||All 13 journal articles|
|Other center views:||All 169 publications||135 publications in selected types||All 122 journal articles|
||Pavlovic J, Hopke PK. Technical note: detection and identification of radical species formed from α-pinene/ozone reaction using DMPO spin trap. Atmospheric Chemistry and Physics Discussions 2009;9(6):23695-23717.||
Supplemental Keywords:RFA, Scientific Discipline, Air, particulate matter, Health Risk Assessment, Environmental Chemistry, Biochemistry, airway epithelial cells, atmospheric particles, cardiopulmonary responses, chemical characteristics, fine particles, airborne particulate matter, aerosol composition, human exposure
Progress and Final Reports:Original Abstract
Main Center Abstract and Reports:R832415 Rochester PM Center
Subprojects under this Center: (EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).
R832415C001 Characterization and Source Apportionment
R832415C002 Epidemiological Studies on Extra Pulmonary Effects of Fresh and Aged Urban Aerosols from Different Sources
R832415C003 Human Clinical Studies of Concentrated Ambient Ultrafine and Fine Particles
R832415C004 Animal models: Cardiovascular Disease, CNS Injury and Ultrafine Particle Biokinetics
R832415C005 Ultrafine Particle Cell Interactions In Vitro: Molecular Mechanisms Leading To Altered Gene Expression in Relation to Particle Composition