Interaction of Chromium and Arsenic in Human Lung Cells and the Role of the Fanc Pathway

EPA Grant Number: F5D40827
Title: Interaction of Chromium and Arsenic in Human Lung Cells and the Role of the Fanc Pathway
Investigators: Savery, Laura C.
Institution: University of Southern Maine
EPA Project Officer: Jones, Brandon
Project Period: September 6, 2005 through September 6, 2008
Project Amount: $96,175
RFA: STAR Graduate Fellowships (2005) RFA Text |  Recipients Lists
Research Category: Academic Fellowships

Objective:

To examine the toxic interactions between chromium and arsenic and determine if co-exposure to arsenic exacerbates the toxic effects of chromium.

Approach:

Integrate toxicogenomic and genetic toxicology approaches using cytotoxicity and uptake assays, chromosome damage assays, comet assays and microarrays to investigate the toxic interactions of chromium and arsenic.

Expected Results:

Arsenic potentiates the carcinogenicity of hexavalent chromium (Cr(VI)) by inhibiting the repair of chromate-induced DNA lesions leading to an increase in chromate-induced chromosomal instability and alterations in gene expression.

Supplemental Keywords:

chromium, arsenic, genotoxicity, chromosome damage, chromosome instability,, Health, Scientific Discipline, Environmental Chemistry, Health Risk Assessment, Risk Assessments, Biochemistry, particulate matter, particle size, chemical exposure, lung injury, chromosome damage assays, cytokines, acute lung injury, nanotechnology, combustion emissions, particle exposure, chemical mixtures, ambient particle health effects, human exposure, respiratory impact, inhalation toxicology, chromium speciation, inhalation study, fanc pathway, biochemical research, human health risk, ultrafine particles, analysis of chemical exposure, arsenic, combination exposure