2001 Progress Report: Urban PM2.5 Surface Chemistry and Interactions with Bronchoalveolar Lavage Fluid (BALF)

EPA Grant Number: R827351C011
Subproject: this is subproject number 011 , established and managed by the Center Director under grant R827351
(EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).

Center: EPA NYU PM Center: Health Risks of PM Components
Center Director: N/A
Title: Urban PM2.5 Surface Chemistry and Interactions with Bronchoalveolar Lavage Fluid (BALF)
Investigators: Kendall, Michaela
Institution: New York University School of Medicine
EPA Project Officer: Chung, Serena
Project Period: June 1, 1999 through May 31, 2005 (Extended to May 31, 2006)
Project Period Covered by this Report: June 1, 2000 through May 31, 2001
RFA: Airborne Particulate Matter (PM) Centers (1999) RFA Text |  Recipients Lists
Research Category: Air Quality and Air Toxics , Particulate Matter , Air

Objective:

The objective of this research project is to examine the surface chemistry of fine particulate matter (PM2.5) collected from concentrated diesel exhaust at roadside urban and rural background locations. In this project, x-ray photon spectroscopy and time-of-flight secondary ion mass spectrometry are used to determine the surface chemistry. Scanning electron microscopy is used to characterize the particles morphologically. The effects of PM surface chemistry and morphology on determining the interactions with human chronic obstructive pulmonary disease (COPD) and normal lavage samples (and components of normal human lavage) then will be quantified, using the same techniques. In addition, enzyme linked immunosorbent assays for specific proteins and a surfactant quantification method will be used to determine lavage component concentrations before and after the introduction of PM.

Progress Summary:

We have established a methodology to: (1) identify specific proteins at PM surfaces; and (2) measure components of lavage fluids at the PM Center and the collaborating research centers.

Table 1. PM2.5 Surface Chemistry Varies

Clean Air PM2.5
Urban PM2.5
Smoke PM2.5
Species
BE (eV)
At %
BE (eV)
At %
BE (eV)
At %
O
532.6
50.4
532.2
10.4
533.0
2.4
NO3-
-
-
407.4
0.7
-
-
NH4+
-
-
402.0
1.5
-
-
N-C
-
-
400.0
1.0
400.0
0.5
C=O/COO
-
-
288.4
6.2
-
-
C-(O,N)
-
-
286.1
12.6
-
-
C-(C,H)
284.6
58.6
284.6
66.5
284.7
96.5
CI=
199.3
11.0
-
-
-
-
SO42-
-
-
168.9
0.5
-
-
SiO2
-
-
101.1
0.6
102.3
0.6

Future Activities:

Future activities are to: (1) complete and interpret zeta-sizer work; (2) analyze surfaces of PM collected in New York City and other sites; (3) identify adsorbent at PM surfaces; and (4) use cell models available at New York University to establish cellular significance of molecular adsorption.

Journal Articles:

No journal articles submitted with this report: View all 2 publications for this subproject

Supplemental Keywords:

time-of-flight secondary ion mass spectrometry, TOF-SIMS, x-ray photon spectroscopy, XPS, scanning electron microscopy, SEM, enzyme linked immunosorbent assays, ELISAs, particulate matter, PM, PM surface chemistry and morphology, chronic obstructive pulmonary disease, COPD., RFA, Health, PHYSICAL ASPECTS, Scientific Discipline, Air, ENVIRONMENTAL MANAGEMENT, particulate matter, Environmental Chemistry, Health Risk Assessment, Risk Assessments, Physical Processes, Environmental Monitoring, Atmospheric Sciences, Risk Assessment, ambient air quality, atmospheric particulate matter, particulates, atmospheric particles, chemical characteristics, toxicology, ambient air monitoring, acute lung injury, airborne particulate matter, environmental risks, exposure, epidemelogy, COPD, lung hypoxia, air pollution, aerosol composition, atmospheric aerosol particles, pulmonary hypertension, human exposure, bronchoalveolar lining fluid, PM, airway contractile properties, exposure assessment

Progress and Final Reports:

Original Abstract
  • 1999
  • 2000
  • 2002 Progress Report
  • 2003 Progress Report
  • 2004
  • Final Report

  • Main Center Abstract and Reports:

    R827351    EPA NYU PM Center: Health Risks of PM Components

    Subprojects under this Center: (EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).
    R827351C001 Exposure Characterization Error
    R827351C002 X-ray CT-based Assessment of Variations in Human Airway Geometry: Implications for Evaluation of Particle Deposition and Dose to Different Populations
    R827351C003 Asthma Susceptibility to PM2.5
    R827351C004 Health Effects of Ambient Air PM in Controlled Human Exposures
    R827351C005 Physicochemical Parameters of Combustion Generated Atmospheres as Determinants of PM Toxicity
    R827351C006 Effects of Particle-Associated Irritants on the Cardiovascular System
    R827351C007 Role of PM-Associated Transition Metals in Exacerbating Infectious Pneumoniae in Exposed Rats
    R827351C008 Immunomodulation by PM: Role of Metal Composition and Pulmonary Phagocyte Iron Status
    R827351C009 Health Risks of Particulate Matter Components: Center Service Core
    R827351C010 Lung Hypoxia as Potential Mechanisms for PM-Induced Health Effects
    R827351C011 Urban PM2.5 Surface Chemistry and Interactions with Bronchoalveolar Lavage Fluid (BALF)
    R827351C012 Subchronic PM2.5 Exposure Study at the NYU PM Center
    R827351C013 Long Term Health Effects of Concentrated Ambient PM2.5
    R827351C014 PM Components and NYC Respiratory and Cardiovascular Morbidity
    R827351C015 Development of a Real-Time Monitoring System for Acidity and Soluble Components in Airborne Particulate Matter
    R827351C016 Automated Real-Time Ambient Fine PM Monitoring System