Regulation of Embryonic Neuronal Development by Chemical Mixtures from Brick, NJ

EPA Grant Number: R829359
Title: Regulation of Embryonic Neuronal Development by Chemical Mixtures from Brick, NJ
Investigators: Reinisch, Carol L. , Kreiling, Jill , Stephens, Raymond
Current Investigators: Reinisch, Carol L. , Cox, Rachel L. , Kreiling, Jill
Institution: Marine Biological Laboratory
EPA Project Officer: Louie, Nica
Project Period: July 1, 2001 through June 30, 2004 (Extended to September 23, 2006)
Project Amount: $749,333
RFA: Complex Chemical Mixtures (2000) RFA Text |  Recipients Lists
Research Category: Hazardous Waste/Remediation , Land and Waste Management , Safer Chemicals

Description:

We will use Spisula as a rapid, reproducible, and exquisitely sensitive model to define the mechanism of action of mixtures of neurotoxic substances on the p53 family of genes during development. We will use bromoform, chloroform, and tectrachloroethylene(PCE), substances of high relevance in the study of autism in Brick, NJ.

Approach:

Define the impact of bromoform/chloroform and / PCE on the p53 family of genes during development. Use amounts of chemicals found in wells in Brick NJ, a site that is the focus of current studies by the EPA. Analyze neuropeptide synthesis and neurospecific protein expression by northern and western blot analysis.

Expected Results:

We expect that mixtures of bromoform, chloroform, and PCE will impact neuronal development and p53 expression in Spisula. By examining the p53 family, we are focusing on the genetic target of chemicals in the environment. Any perturbations in p53 in any species should serve as a critically important biomarker for chemical exposure. We have selected chemical mixtures which impair neural function. Together, these data should have relevance to state and federal agencies responsible for risk assessment and management.

Publications and Presentations:

Publications have been submitted on this project: View all 2 publications for this project

Journal Articles:

Journal Articles have been submitted on this project: View all 1 journal articles for this project

Supplemental Keywords:

biomarkers, brain function, neurotransmitters., RFA, Scientific Discipline, Waste, Geographic Area, Environmental Chemistry, State, chemical mixtures, Fate & Transport, Hazardous Waste, Ecology and Ecosystems, Hazardous, complex mixtures, contaminated sediments, fate and transport, embryonic neuronal development, fate and transport , effects assessment, biodegradation, bioavailability, hazardous organic substances, New Jersey (NJ), environmental transport and fate, chemical kinetics, genotoxicity, hazardous chemicals, PCE, contaminated soils, analytical models

Progress and Final Reports:

  • 2002
  • 2003 Progress Report
  • 2004 Progress Report
  • 2005
  • 2006
  • Final