Regulation of Embryonic Neuronal Development by Chemical Mixtures from Brick, NJEPA Grant Number: R829359
Title: Regulation of Embryonic Neuronal Development by Chemical Mixtures from Brick, NJ
Investigators: Reinisch, Carol L. , Kreiling, Jill , Stephens, Raymond
Current Investigators: Reinisch, Carol L. , Cox, Rachel L. , Kreiling, Jill
Institution: Marine Biological Laboratory
EPA Project Officer: Louie, Nica
Project Period: July 1, 2001 through June 30, 2004 (Extended to September 23, 2006)
Project Amount: $749,333
RFA: Complex Chemical Mixtures (2000) RFA Text | Recipients Lists
Research Category: Hazardous Waste/Remediation , Land and Waste Management , Safer Chemicals
We will use Spisula as a rapid, reproducible, and exquisitely sensitive model to define the mechanism of action of mixtures of neurotoxic substances on the p53 family of genes during development. We will use bromoform, chloroform, and tectrachloroethylene(PCE), substances of high relevance in the study of autism in Brick, NJ.
Define the impact of bromoform/chloroform and / PCE on the p53 family of genes during development. Use amounts of chemicals found in wells in Brick NJ, a site that is the focus of current studies by the EPA. Analyze neuropeptide synthesis and neurospecific protein expression by northern and western blot analysis.
We expect that mixtures of bromoform, chloroform, and PCE will impact neuronal development and p53 expression in Spisula. By examining the p53 family, we are focusing on the genetic target of chemicals in the environment. Any perturbations in p53 in any species should serve as a critically important biomarker for chemical exposure. We have selected chemical mixtures which impair neural function. Together, these data should have relevance to state and federal agencies responsible for risk assessment and management.