Solid-Matrix Luminescence Analysis of B[a]P-DNA AdductsEPA Grant Number: R824100
Title: Solid-Matrix Luminescence Analysis of B[a]P-DNA Adducts
Investigators: Hurtubise, Robert J.
Institution: University of Wyoming
EPA Project Officer: Hiscock, Michael
Project Period: October 1, 1995 through September 30, 1998
Project Amount: $431,400
RFA: Exploratory Research - Chemistry and Physics of Water (1995) RFA Text | Recipients Lists
Research Category: Water , Land and Waste Management , Engineering and Environmental Chemistry
Description:The main objectives of this research are to develop a basic understanding of the physicochemical interactions of benzo[a]pyrene (B[a]P)-DNA adducts, small molecular weight adducts, and B[a]P metabolites adsorbed on a variety of solid matrices. The principles developed will be related to the luminphor-solid matrix interactions that are important for improving the sensitivity and selectivity of solid-matrix luminescence analysis. To clarify the physicochemical interactions, emphasis will be on heavy-atom effects, temperature and humidity changes, and unique solid matrices such as hydrophobic paper. Another goal of the project is to develop capillary electrophoresis into an effective tool for the separation of B[a]P-DNA adducts from small molecular weight adducts and metabolites. Also, laser-induced fluorescence will be combined with capillary electrophoresis into an effective tool to directly detect and quantitate B[a]P-DNA adducts and their metabolites in the capillary system.
Solid-matrix luminescence will be used to uniquely characterize, in detail, capillary electrophoretic fractions containing B[a]P-DNA adducts, small molecular weight adducts, and metabolites.
The results of this research will give a better understanding of the significant interactions needed to enhance the sensitivity and selectivity of solid-matrix luminescence. Furthermore, by combining the methodologies of solid-matrix luminescence, capillary electrophoresis, and laser-induced fluorescence, sensitive and selective analytical methods for B[a]P-DNA adducts and related small molecular weight species will be developed. These methods will be applicable to a number of important samples related to environmental testing, such as fish samples and samples for human population monitoring for B[a]P-DNA adducts and their metabolites.