Catalytic Function of Expressed Teleost Cytochrome P4501A

EPA Grant Number: R827102
Title: Catalytic Function of Expressed Teleost Cytochrome P4501A
Investigators: Stegeman, John J.
Institution: Woods Hole Oceanographic Institution
EPA Project Officer: Manty, Dale
Project Period: September 1, 1995 through August 1, 1996
Project Amount: $251,946
RFA: Exploratory Research - Environmental Biology (1995) RFA Text |  Recipients Lists
Research Category: Biology/Life Sciences , Health , Ecosystems

Description:

The purpose of this grant is to establish in an unambiguous way, the catalytic properties of the cytochrome P4501A protein in fish. This protein is induced (i.e., increased in amount) in many organs of fish exposed to polynuclear aromatic hydrocarbons and planar halogenated aromatic hydrocarbons in the environment. This induction is the most robust indicator (biomarker) of exposure to such compounds. Knowing the exogenous and endogenous substrates metabolized by the CYP1A1 will greatly improve the interpretation of the biological significance of that induction. To obtain this information, the gene for CYP1A1 was cloned from the marine fish scup, and the gene was then incorporated into a cell line that does not express other P450s. The ability of the scup CYP1A1 made in these cells to metabolize a variety of candidate and diagnostic substrates is being examined, without the interference by other P450s. This is difficult, if not impossible to do, with preparations of animal tissues, which express other P450s in addition to CYP1A1.

Publications and Presentations:

Publications have been submitted on this project: View all 18 publications for this project

Journal Articles:

Journal Articles have been submitted on this project: View all 17 journal articles for this project

Supplemental Keywords:

Health, Scientific Discipline, Ecology, Environmental Chemistry, Chemistry, Risk Assessments, Biology, diagnostic substrates, catalytic function, hydrocarbon, animal model, genetic analysis, genetic engineering, fish proteins, cytochrome P4501A, biomarker, genetic susceptibility

Progress and Final Reports:

1999 Progress Report
Final Report