Aerobic Cometabolism of Chlorinated Aliphatic Hydrocarbon Compounds with Butane-Grown Microorganisms

EPA Grant Number: R828772C003
Subproject: this is subproject number 003 , established and managed by the Center Director under grant R828772
(EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).

Center: HSRC (2001) - Western Region Hazardous Substance Research Center for Developing In-Situ Processes for VOC Remediation in Groundwater and Soils
Center Director: Semprini, Lewis
Title: Aerobic Cometabolism of Chlorinated Aliphatic Hydrocarbon Compounds with Butane-Grown Microorganisms
Investigators: Arp, Daniel J. , Bottomley, Peter , Ciuffetti, Lynda , Giovannoni, Stephen , Semprini, Lewis , Williamson, Kenneth J.
Current Investigators: Arp, Daniel J. , Bottomley, Peter , Ciuffetti, Lynda , Dolan, Mark E. , Williamson, Kenneth J.
Institution: Oregon State University
EPA Project Officer: Lasat, Mitch
Project Period: June 1, 2001 through September 30, 2006
Project Amount: Refer to main center abstract for funding details.
RFA: Hazardous Substance Research Centers - HSRC (2001) RFA Text |  Recipients Lists
Research Category: Hazardous Waste/Remediation , Land and Waste Management

Objective:

Aerobic cometabolism offers a biological alternative for the removal of pollutants from contaminated aquifers. This approach to bioremediation is particularly appropriate to chlorinated aliphatic hydrocarbons (CAHs) where other methods are less likely to result in the complete mineralization of the target compound. However, use of aerobic cometabolism in the field requires a thorough understanding of the factors that limit cometabolism. This proposal addresses three areas where current knowledge is inadequate. The specific objectives are: 1) Identification of culture conditions that maximize CAH degradation potential by individual strains and a well-characterized mixed culture of butane oxidizing bacteria (BGM1). 2) Long-term studies of the sustainability of BGM1-supported CAH transformation in batch and continuous flow column studies. Aerobic cometabolism offers a biological alternative for the removal of pollutants from contaminated aquifers. This approach to bioremediation is particularly appropriate to chlorinated aliphatic hydrocarbons (CAHs) where other methods are less likely to result in the complete mineralization of the target compound. However, use of aerobic cometabolism in the field requires a thorough understanding of the factors that limit cometabolism. This proposal addresses three areas where current knowledge is inadequate. The specific objectives are: 1) Identification of culture conditions that maximize CAH degradation potential by individual strains and a well-characterized mixed culture of butane oxidizing bacteria (BGM1). 2) Long-term studies of the sustainability of BGM1-supported CAH transformation in batch and continuous flow column studies.

Approach:

Cometabolism requires specific growth substrates. In these experiments, we will use individual strains and BGM1 grown on butane. The pure cultures were selected because they are known to grow on butane and simultaneously degrade CAHs. The mixed culture, BGM1, is a well-characterized culture that is destined for use in field trials at Moffett Air Field. Degradation potential will be measured in batch cultures grown under various conditions. Viability (plate counts) and other indicators of cell health (e.g. O2 uptake) will be determined, as will rates and sustainability of CAH degradation. Small columns (3 cm x 20 cm) will be inoculated and run in batch mode. The columns will be filled with aquifer material and inoculated with either individual strains or BGM1. Larger columns (5 cm x 40 cm) will be run in continuous mode. Composition of bacterial communities will be determined with LH-PCR and with strain specific molecular probes. Columns will be fed butane and challenged with CAHs.

Expected Results:

Information about the influence of growth conditions on the rates and sustainability of aerobic cometabolism by well-characterized individual strains and BGM1 will be obtained. This information will guide development of column experiments which are expected to mimic the field site and provide information about the movement of microorganisms in the aquifer material, the growth and persistence of the bacteria, and the sustainability of aerobic cometabolism (Objective 2). Methods will be developed for tracking the movement and composition of BGM1 as CAH degradation proceeds. The results, including model development, will improve the design and efficacy of cometabolism-based remediation strategies.

Publications and Presentations:

Publications have been submitted on this subproject: View all 3 publications for this subprojectView all 158 publications for this center

Journal Articles:

Journal Articles have been submitted on this subproject: View all 2 journal articles for this subprojectView all 60 journal articles for this center

Supplemental Keywords:

cometabolism, cooxidation, chlorinated aliphatic compounds, butane, propane, length heterogeneity, bacterial stress, RFA, Scientific Discipline, Geographic Area, Waste, TREATMENT/CONTROL, Ecosystem Protection/Environmental Exposure & Risk, Sustainable Industry/Business, Bioavailability, cleaner production/pollution prevention, Sustainable Environment, Remediation, Treatment Technologies, Technology for Sustainable Environment, Hazardous Waste, New/Innovative technologies, Bioremediation, Environmental Engineering, Urban and Regional Planning, Hazardous, EPA Region, region 4, contaminated sediments, hazardous substance disposal, fate, fate and transport, fate and transport , contaminated sediment, aliphatic compounds, aerobic cometabolism, Region 6, dredging, butane, outreach and education, pollution prevention, technology transfer, phytoremediation, contaminated aquifers, chlorinated aliphatic hydrocarbons

Progress and Final Reports:

  • 2001
  • 2002 Progress Report
  • 2003 Progress Report
  • 2004
  • 2005
  • Final

  • Main Center Abstract and Reports:

    R828772    HSRC (2001) - Western Region Hazardous Substance Research Center for Developing In-Situ Processes for VOC Remediation in Groundwater and Soils

    Subprojects under this Center: (EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).
    R828772C001 Developing and Optimizing Biotransformation Kinetics for the Bio- remediation of Trichloroethylene at NAPL Source Zone Concentrations
    R828772C002 Strategies for Cost-Effective In-situ Mixing of Contaminants and Additives in Bioremediation
    R828772C003 Aerobic Cometabolism of Chlorinated Aliphatic Hydrocarbon Compounds with Butane-Grown Microorganisms
    R828772C004 Chemical, Physical, and Biological Processes at the Surface of Palladium Catalysts Under Groundwater Treatment Conditions
    R828772C005 Effects of Sorbent Microporosity on Multicomponent Fate and Transport in Contaminated Groundwater Aquifers
    R828772C006 Development of the Push-Pull Test to Monitor Bioaugmentation with Dehalogenating Cultures
    R828772C007 Development and Evaluation of Field Sensors for Monitoring Bioaugmentation with Anaerobic Dehalogenating Cultures for In-Situ Treatment of TCE
    R828772C008 Training and Technology Transfer
    R828772C009 Technical Outreach Services for Communities (TOSC) and Technical Assistance to Brownfields Communities (TAB) Programs
    R828772C010 Aerobic Cometabolism of Chlorinated Ethenes by Microorganisms that Grow on Organic Acids and Alcohols
    R828772C011 Development and Evaluation of Field Sensors for Monitoring Anaerobic Dehalogenation after Bioaugmentation for In Situ Treatment of PCE and TCE
    R828772C012 Continuous-Flow Column Studies of Reductive Dehalogenation with Two Different Enriched Cultures: Kinetics, Inhibition, and Monitoring of Microbial Activity
    R828772C013 Novel Methods for Laboratory Measurement of Transverse Dispersion in Porous Media
    R828772C014 The Role of Micropore Structure in Contaminant Sorption and Desorption