Electrochemical Enzyme Immunoassay to Study Alternative Drinking Water Treatment

EPA Contract Number: 68D00031
Title: Electrochemical Enzyme Immunoassay to Study Alternative Drinking Water Treatment
Investigators: O'Daly, John P.
Small Business: Enzyme Technology Research Group Inc.
EPA Contact: Manager, SBIR Program
Phase: I
Project Period: August 1, 1990 through February 1, 1991
Project Amount: $49,978
RFA: Small Business Innovation Research (SBIR) - Phase I (1991) RFA Text |  Recipients Lists
Research Category: Water and Watersheds , SBIR - Water and Wastewater , Small Business Innovation Research (SBIR)


Alternatives to chlorine disinfection in drinking water treat- ment are being investigated. Both current and new methods of disinfection may cause reactions with organic contami- nants in the water and lead to dangerous by-products. There is a need for measurement devices that provide on-site, real- time measurement of by-products to assist in the develop- ment of alternate water treatment methods. For this purpose, the researchers propose to evaluate and develop a new biosensor design that combines advantages of immunoas- say and electrochemical response. The specific objective of the Phase I work is to prove the principle of electrochemical enzyme immunosensors to detect organic by-product for- mation in water. The proposed biosensor consists of three major elements; an electrical conducting layer having im- mobilized enzyme, a gel layer containing polyclonal or monoclonal antibody and other necessary reagents, and the electronic components used in the readout of the signal. The expected result is an immunoelectrode for measuring or- ganics in water, based on the principal of coupling the immunochemical reaction to the electrode response using an electrochemically active mediator. The three researchers who submitted this proposal have collaborated successfully to develop prototype enzyme-based biosensor technology which will be a crucial component for the research and development described in this proposal. Practical electro- chemical sensors that employ immunochemical detection will have broad commercial viability for applications in the fields of clinical chemistry, veterinary medicine, agriculture, environmental monitoring, and pollution control.

Supplemental Keywords:

RFA, Scientific Discipline, Toxics, Water, Ecosystem Protection/Environmental Exposure & Risk, Sustainable Industry/Business, Chemical Engineering, Environmental Chemistry, Sustainable Environment, Chemistry, Technology for Sustainable Environment, Monitoring/Modeling, Analytical Chemistry, Biochemistry, Drinking Water, Engineering, EPCRA, Engineering, Chemistry, & Physics, Environmental Engineering, alternative disinfection methods, immunoassay, electrochemical enzyme, biosensing, electrically driven enzymes, Chlorine, chlorinated by products, disinfection by-products, real time, enzymes, immunological assay, chlorinated DBPs, electrochemical enzyme, treatment, disinfection byproducts (DBP), electrochemical techniques, chlorine-based disinfection, chlorine disinfection, electrochemical, electrochemical enzymes, chlorine disinfection alternatives, measurement, DBP risk management, biosensor, biosensors, other - risk management

Progress and Final Reports:

  • Final