Development of an Immunobiosensor for Detection of PCBs

EPA Contract Number: 68D60022
Title: Development of an Immunobiosensor for Detection of PCBs
Investigators: Ray, Bryan L.
Small Business: SymBiotech Inc.
EPA Contact: Manager, SBIR Program
Phase: I
Project Period: September 1, 1996 through March 1, 1997
Project Amount: $70,000
RFA: Small Business Innovation Research (SBIR) - Phase I (1996) RFA Text |  Recipients Lists
Research Category: Ecological Indicators/Assessment/Restoration , SBIR - Monitoring , Small Business Innovation Research (SBIR)

Description:

This proposal aims to develop an immuno-based biosensor for detecting PCBs in soil at 50ng/g and in water at 1 ng/L when combined with a sample concentration method to be developed in this proposal. The immunobiosensor will employ a photodetection system comprising bacteriorhodopsin, an integral membrane protein of the purple membrane of Halobacterium halobium. Bacteriorhodopsin pumps protons across the membrane when exposed to light. This response to light can be electronically monitored when purple membranes are deposited onto an electrode. Specific affinity interactions of antibodies with haptens (e.g., PCBs) can alter the photoresponse of these purple membranes. The ability of antibody/hapten inter-actions to quantitatively alter this photoresponse forms the basis for the immunobiosensor technology. The feasability of this technology will be determined.

Supplemental Keywords:

small business, SBIR, engineering, chemistry, immunoassay, hazardous waste, RFA, Scientific Discipline, Toxics, Waste, Water, Ecosystem Protection/Environmental Exposure & Risk, Contaminated Sediments, Environmental Chemistry, pesticides, Monitoring/Modeling, Hazardous Waste, Environmental Monitoring, Hazardous, Environmental Engineering, biosensing, monitoring, PCB detoxification, polychlorinated biphenyls (PCBs), soil sediment, immunobiosensor, contaminated soil, analytical chemistry, biosensing system, soil, PCB, bioassay, pesticide residue, hazardous chemicals, immunobisensor, biosensors, biosensor, membrane technology

Progress and Final Reports:

  • Final